Immunofluorescence analysis of HeLa cells, labeling Eg5 using ab171963 at a 1/100 dilution (red), and DAPI nuclear staining (blue).
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate, labeling Eg5 using ab171963 at a 1/10 dilution, and an IgG control.
![All lanes : Anti-Eg5 antibody [EPR12280] (ab171963) at 1/1000 dilutionLane 1 : Raji cell lysateLane 2 : HeLa cell lysateLane 3 : K-562 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP labeled goat anti-rabbit at 1/2000 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/12059_ab171963-172656-ab171963wb.jpg)
All lanes : Anti-Eg5 antibody [EPR12280] (ab171963) at 1/1000 dilutionLane 1 : Raji cell lysateLane 2 : HeLa cell lysateLane 3 : K-562 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP labeled goat anti-rabbit at 1/2000 dilutiondeveloped using the ECL technique