![All lanes : Anti-Eph receptor B1 antibody [EPR6457] (ab129103) at 1/1000 dilutionLane 1 : MDA-MB-435 cell lysateLane 2 : U-87MG cell lysateLane 3 : NIH 3T3 cell lysateLane 4 : COS1 cell lysateLane 5 : Human fetal brain cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/14287_Eph-receptor-B1-Primary-antibodies-ab129103-2.jpg)
All lanes : Anti-Eph receptor B1 antibody [EPR6457] (ab129103) at 1/1000 dilutionLane 1 : MDA-MB-435 cell lysateLane 2 : U-87MG cell lysateLane 3 : NIH 3T3 cell lysateLane 4 : COS1 cell lysateLane 5 : Human fetal brain cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution
Immunofluorescence analysis of rat oligodendrocytes, staining Eph receptor B1 with ab129103. Cells were fixed with formaldehyde, permeabilized with 0.03% Triton X-100 and blocked with 1% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/300 in serum + 0.01% Triton X-100) for 10 hours at 4°C. An AlexaFluor®555-conjugated donkey anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody. See Abreview
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD