Anti-FXYD1 antibody [EPR16082]

Name	Anti-FXYD1 antibody [EPR16082]
Supplier	Abcam
Catalog	ab200204
Prices	$347.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR16082
Applications	WB IP
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FXYD1 aa 50 to the C-terminus
Description	Rabbit Monoclonal
Gene	FXYD1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Anti-FXYD1 antibody [EPR16082] (ab200204) at 1/1000 dilution + Human skeletal muscle lysate at 20 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-FXYD1 antibody [EPR16082] (ab200204) at 1/10000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human fetal liver lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-FXYD1 antibody [EPR16082] (ab200204) at 1/1000 dilutionLane 1 : Mouse heart lysateLane 2 : Rat heart lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

FXYD1 was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab200204 at 1/120 dilution. Western blot was performed using ab200204 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Human fetal heart whole cell lysate 10ug (Input).Lane 2: ab200204 IP in Human fetal heart whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200204 in Human fetal heart whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.