![All lanes : Anti-Histone H2A.Z antibody [IC10] (ab4462) at 10 µg/mlLane 1 : Histone H2A.Z recombinant proteinLane 2 : Histone H2A recombinant proteinLysates/proteins at 0.5 µg per lane.SecondaryGoat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilutionPerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/1240_Histone-H2AZ-Primary-antibodies-ab4462-6.jpg)
All lanes : Anti-Histone H2A.Z antibody [IC10] (ab4462) at 10 µg/mlLane 1 : Histone H2A.Z recombinant proteinLane 2 : Histone H2A recombinant proteinLysates/proteins at 0.5 µg per lane.SecondaryGoat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilutionPerformed under reducing conditions.
ab4462 (1/1000) staining Histone H2A.Z in paraffin-embedded Human pancreatic cancer tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. After the primary antibody incubation a rabbit anti-rat link was used before the application of the secondary antibody which was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.See Abreview