
All lanes : Anti-Hsp60 antibody (ab137706) at 1/10000 dilutionLane 1 : H1299 whole cell lysateLane 2 : HeLa S3 whole cell lysateLane 3 : MOLT4 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Hsp60 antibody (ab137706) at 1/20000 dilution + Mouse brain whole cell lysate at 30 µg

Immunohistochemistry analysis of paraffin-embedded NCI-N87 xenograft tissue labelling Hsp60 with ab137706 at 1/500.

Immunofluorescence analysis of methanol-fixed A431 cells labelling Hsp60 with ab137706 at 1/200 (panel 1) and co-stained with Hoechst 33342 (panel 2).