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All lanes : Anti-JunB (phospho S259) antibody (ab30628) at 1/500 dilutionLane 1 : extracts from HeLa cells (5-30ug).Lane 2 : extracts from HeLa cells (5-30ug), preincubated with synthesized non phosphopeptide.Lane 3 : extracts from HeLa cells (5-30ug), preincubated with synthesized phosphopeptide (negative control).
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Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 JunB (Phospho Ser259) Antibody (ab30628). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).