
Flow cytometric analysis of permeabilized Jurkat cells labeling NFAT2 (red) with ab177464 at 1/10 dilution, or a rabbit IgG (negative) (green).

Immunofluorescence analysis of Raji cells labeling NFAT2 (green) with ab177464 at 1/50 dilution. DAPI nuclear staining (blue).
![All lanes : Anti-NFAT2 antibody [EPR2431(N)(B)] (ab177464) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : Raji cell lysateLane 3 : Ramos cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/29861_ab177464-205797-wb.jpg)
All lanes : Anti-NFAT2 antibody [EPR2431(N)(B)] (ab177464) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : Raji cell lysateLane 3 : Ramos cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique