![All lanes : Anti-RFC3 antibody [EPR14214] (ab182143) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/22347_ab182143-213975-ab1821431.jpg)
All lanes : Anti-RFC3 antibody [EPR14214] (ab182143) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling RFC3 using ab182143 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

Flow Cytometry analysis of HeLa cells labeling RFC3 using ab182143 at a 1/250 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde