![All lanes : Anti-RING1 antibody [8E8A2] (ab181756) at 1/500 dilutionLane 1 : HEK293 cell lysate, non-transfectedLane 2 : HEK293 cell lysate, transfected with RING1 (aa 79-263)-hIgGFc](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/23311_ab181756-211064-ab181756b.jpg)
All lanes : Anti-RING1 antibody [8E8A2] (ab181756) at 1/500 dilutionLane 1 : HEK293 cell lysate, non-transfectedLane 2 : HEK293 cell lysate, transfected with RING1 (aa 79-263)-hIgGFc
![Anti-RING1 antibody [8E8A2] (ab181756) at 1/500 dilution + Human RING1 recombinant protein](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/23312_ab181756-211063-ab181756.jpg)
Anti-RING1 antibody [8E8A2] (ab181756) at 1/500 dilution + Human RING1 recombinant protein

Immunofluorescent analysis of HeLa cells labeling RING1 with ab181756 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunofluorescent analysis of A431 cells labeling RING1 with ab181756 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.