
All lanes : Anti-RRAS antibody (ab82356) at 1/250 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HT29 (Human colon adenocarcinoma) Whole Cell LysateLane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 4 : DU 145 (Human prostate carcinoma cell line) Whole Cell LysateLane 5 : PC3 (Human prostate cancer cell line) Whole Cell Lysate (ab3954)Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.