
Anti-RRM2 antibody - Azide free (ab191800) at 1/1000 dilution + MCF7 whole cell extract at 20 µg

Anti-RRM2 antibody - Azide free (ab191800) at 1/2000 dilution + HeLa whole cell extarct at 20 µg

Anti-RRM2 antibody - Azide free (ab191800) at 1/1000 dilution + Mitotic extract of Xenopus egg

MCF7 cells were fixed with 4% PFA and permeabilized with 0.25% TritonX 100 and stained with ab191800 at a 1/100 dilution. As the secondary antibody, Alexa Fluor® 488 conjugated anti-rabbit IgG antibody was used at 1/1000 dilution. The sample was counter stained with 1.0 μg/mL DAPI in TBS.