
RM149 specifically reacts to Histone H3 acetylated
at Lysine 4 (K4ac). No cross reactivity with non-
modified Lysine 4, acetylated Lysine 9 (K9ac), Lysine
14 (K14ac), Lysine 18 (K18ac),Lysine 23 (K23ac),
Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56
(K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in
histone H3.

Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
using RM149 at 0.5 ug/mL, showed a band of
histone H3 acetylated at Lysine 4 in treated
HeLa.

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys4)
Rabbit mAb RM149 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).