RM166 specifically reacts to Histone H3
acetylated at Lysine 18 (K18ac). No cross reactivity
with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac),
Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27
(K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac),
Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.
Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
using RM166 at 0.5 ug/mL, showed a band of
histone H3 acetylated at Lysine 18 in treated
HeLa.
Sandwich ELISA against acetylated histone H3
at Lys 18 using HeLa whole cell lysate, treated or
untreated with Sodium Butyrate. Using anti-H3CT
(RM188, 1 ug/mL) as the capture antibody and
biotinylated anti-H3K18C (RM166, 2 ug/mL) as the
detection antibody.
ChIP performed on HeLa cells with or without
Sodium Butyrate treatment, using H3K18Ac
antibody (RM166, 5ug). Real-time PCR was
performed using primers specific to the gene
indicated.
Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys18)
Rabbit mAb RM166(red). Actin filaments have
been labeled with fluorescein phalloidin (green).
Immunohistochemistry staining of HepG2 cells using
Anti-Acetyl-Histone H3 (Lys18) antibody, RM166.