
RM235 specifically reacts to Histone H2B acetylated
at Lysine 20 (K20ac). No cross reactivity with non-
modified Lysine 20 or other acetylated Lysines in
histone H2B.

Western Blot of acid extracts from HeLa cells
treated (+) or untreated (-) with sodium butyrate,
using RM235 at 0.5 ug/mL.

Immunocytochemical staining of HeLa cells treated
with sodium butyrate, using anti-Acetyl-Histone
H2B (Lys20) Rabbit Monoclonal Antibody (clone
RM235) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).