Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with KLF4 antibody (N-term C74) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with KLF4 antibody (N-term C74) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent confocal image of SY5Y cells stained KLF4 (N-term C74) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 (N-term C74) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). KLF4 immunoreactivity is localized to the nuclei in SY5Y cells.
Fluorescent confocal image of HeLa cells stained KLF4 (N-term C74) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 (N-term C74) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). KLF4 immunoreactivity is localized to the nuclei in HeLa cells.
Western blot of KLF4 Antibody (N-term C74) in Jurkat,293 cell line lysates (35 ug/lane). KLF4 (arrow) was detected using the purified antibody.
Western blot of KLF4 Antibody (N-term C74) in mouse stomach tissue lysates (35 ug/lane). KLF4 (arrow) was detected using the purified antibody.