HMGB1/HMG-1 Antibody

Name	HMGB1/HMG-1 Antibody
Supplier	Novus Biologicals
Catalog	NB100-2322
Prices	$129.00, $339.00
Sizes	25 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB Simple Western ELISA FC ICC/IF IHC IHC-P
Species Reactivities	Human, Rat, Bovine, Cat, Rabbit, Sheep, Horse
Antigen	A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). [UniProt #P09429]
Purity/Format	Immunogen affinity purified
Blocking Peptide	HMGB1/HMG-1 Blocking Peptide
Description	Rabbit Polyclonal
Gene	HMGB1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - Total protein from SHSY-5Y, MCF7, Neuro2A and HeLa was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HMGB1 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody [NB100-2322] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-HMGB1 [NB100-2322] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody [NB100-2322] - Staining of HMGB1 in mouse liver using NB100-2322.

Flow Cytometry: HMGB1/HMG-1 Antibody [NB100-2322] - Staining of NTERA-2 cells using NB100-2322 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody.

Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - Detection of HMGB1 in whole cell HeLa lysate using NB100-2322. ECL exposure, 1 min.

Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - HMGB1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A concentration of 0.05 ug/ml was used. Image objective 40x.

Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - Hepatocyte protein lysate at 1:1000 4C overnight. Image from verfified customer review.

Simple Western: HMGB1/HMG-1 Antibody [NB100-2322] - Simple Western lane view shows a specific band for HMGB1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

ELISA: HMGB1/HMG-1 Antibody [NB100-2322] - A dose-dependent titration of the HRP conjugated anti-HMGB1 antibody (Catalog Number: NB100-2322H) on recombinant human HMGB1 protein. Image from verified customer review.