Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Perilipin-2/ADFP at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - Detection of ADFP (Green) in Hela cells using NB110-40877 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.

Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - ADFP antibody was tested in mouse liver using DAB with hematoxylin counterstain.

Simple Western: Perilipin-2/ADFP Antibody [NB110-40877] - Simple Western lane view shows a specific band for ADFP in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.