Recombinant Porcine TNF‑ alpha (Catalog #690‑PT) induces cytotoxicity in the the PK‑15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Porcine TNF‑ alpha (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF690). The ND50 is typically 0.2-0.8 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
TNF‑ alpha was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Goat Anti-Porcine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF690) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.