Western blot analysis of extracts from ACHN and HeLa cells using Smad2/3 Antibody.
Confocal immunofluorescent analysis of HT-1080 cells, serum-starved (left), treated with Human TGF-β1 #8915 (1 μg/mL for 30 min; center) or treated with Human TGF-β1 and SB43152 (10 μg/mL for 30 min; right), using Smad2/Smad3 Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red).
Flow cytometric analysis of HT-1080 cells using Smad2/3 Antibody (blue) compared to a Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HaCat cells treated with Human TGF-β3 #3706 (7 ng/ml) for 1 h and either 10 μl of Smad2/3 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human CDKN1A Intron 1 Primers #4669, SimpleChIP ® Human ID1 Promoter Primers #5139, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.