Western blot analysis of extracts from 293 cells, transfected with wild-type Tie2 (lane 1) or mock-transfected (lane 2), using Phospho-Tie2 (Tyr992) Antibody. Tie2 is constitutively active when transfected into 293 cells.
Western blot analysis of extracts from Sf9 cells over expressing GST-human Tie2 kinase domain fusion proteins, wild-type (lane 1) or kinase-dead (lane 2), using Phospho-Tie2 (Tyr992) Antibody (upper) or Tie2 antibody (lower). The wild-type Tie2 kinase domain is constitutively phosphorylated when overexpressed in Sf9 cells. The molecular weight of GST-Tie2 fusion is approximately 65 kDa.