![Immunoprecipitation: lin-54 Antibody [NBP2-14854] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-LIN54 antibody used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. LIN54 was also immunoprecipitated by rabbit anti-LIN54 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/12605_lin-54-Antibody-Immunoprecipitation-NBP2-14854-img0001.jpg)
Immunoprecipitation: lin-54 Antibody [NBP2-14854] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-LIN54 antibody used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. LIN54 was also immunoprecipitated by rabbit anti-LIN54 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).