Anti-BMP11 antibody [EPR4567(2)] (ab124721) at 1/5000 dilution (purified) + HeLa cell lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-BMP11 antibody [EPR4567(2)] (ab124721) at 1/1000 dilution (purified) + C6 cell lysate at 20 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-BMP11 antibody [EPR4567(2)] (ab124721) at 1/1000 dilution (unpurified)Lane 1 : HeLa cell lysateLane 2 : Human heart tissue lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling BMP11 with purified ab124721 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling BMP11 with unpurified ab124721 at 1/500 dilution.

Immunocytochemistry/Immunofluorescence analysis of A549 cells labelling BMP11 with purified ab124721 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. ab150078, an Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/250) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD