![All lanes : Anti-DNA Ligase IV antibody [EPR16531] (ab193353) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : Ramos cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/8963_ab193353-230970-ab1933531.jpg)
All lanes : Anti-DNA Ligase IV antibody [EPR16531] (ab193353) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : Ramos cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin embedded Human thymus tissue sections labeling DNA Ligase IV using ab193353 at a 1/50 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Immunofluorescent analysis of 4% paraformaldehyde fixed SH-SY5Y cells labeling DNA Ligase IV using ab193353 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.