Human DNA PKcs peptide

Name	Human DNA PKcs peptide
Supplier	Abcam
Catalog	ab20407
Category	Peptide
Nature	Synthetic
SwissProt/Accession	P78527
Gene	PRKDC
Sequence	Belongs to the PI3/PI4-kinase family. Contains 1 FAT domain. Contains 1 FATC domain. Contains 2 HEAT repeats. Contains 1 PI3K/PI4K domain. Contains 3 TPR repeats.
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Product images

All lanes : Anti-DNA PKcs (phospho S2056) antibody - ChIP Grade ( ab18192 ) at 1 µg/ml Lane 1 : HeLa Gamma Irradiated Whole Cell Lysate Pack ( ab13823 ) at 20 µg Lane 2 : 20ug of untreated HeLa cell extract Lane 3 : HeLa Gamma Irradiated Whole Cell Lysate Pack ( ab13823 ) at 20 µg with Human DNA PKcs (phospho S2056) peptide ( ab20406 ) at 1 µg/ml Lane 4 : 20ug of untreated HeLa cell extract with Human DNA PKcs (phospho S2056) peptide ( ab20406 ) at 1 µg/ml Lane 5 : HeLa Gamma Irradiated Whole Cell Lysate Pack ( ab13823 ) at 20 µg with Human DNA PKcs peptide (ab20407) at 1 µg/ml Lane 6 : 20ug of untreated HeLa cell extract with Human DNA PKcs peptide (ab20407) at 1 µg/ml Secondary Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution Performed under reducing conditions. Observed band size : 460 kDa ( why is the actual band size different from the predicted? ) Additional bands at : 270 kDa (possible cleavage fragment,cross reactivity).