All lanes : Anti-CNPase antibody ( ab27695 ) at 1 µg/ml Lane 1 : Human brain lysate Lane 2 : Mouse brain lysate Lane 3 : Rat brain lysate Lane 4 : Human brain lysate with Human CNPase peptide (ab19424) at 1 µg/ml Lane 5 : Mouse brain lysate with Human CNPase peptide (ab19424) at 1 µg/ml Lane 6 : Rat brain lysate with Human CNPase peptide (ab19424) at 1 µg/ml Lysates/proteins at 20 µg per lane. Secondary Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution Performed under reducing conditions. Predicted band size : 46, 48 kDa