PFTAIRE-1 Antibody (C-3): sc-376366

PFTAIRE-1 Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PFTAIRE-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-PFTAIRE-1 antibody (C-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PFTAIRE-1 plays a crucial role in cell cycle regulation, particularly in the transition from G1 phase to S phase, by interacting with various cyclin-dependent kinases (CDKs). Such interaction is vital for proper cell division and proliferation, making PFTAIRE-1 an important target for studies related to cancer and other proliferative diseases. PFTAIRE-1 is localized in the cytoplasm of HeLa cells, despite possessing two N-terminal nuclear localization sequences, suggesting a complex regulatory mechanism governing function and localization. Understanding PFTAIRE-1 dynamics and interactions with other proteins, such as Cdk3, Cdk4, and Cdk6, can provide insights into molecular pathways that control cell cycle progression and potential implications in tumorigenesis.

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PFTAIRE-1 Antibody (C-3) References:

1. Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.  |  Nagase, T., et al. 1998. DNA Res. 5: 355-64. PMID: 10048485
2. Identification and cellular localization of human PFTAIRE1.  |  Yang, T. and Chen, JY. 2001. Gene. 267: 165-72. PMID: 11313143
3. A novel TRH-PFTAIRE protein kinase 1 pathway in the cerebellum: subtractive hybridization analysis of TRH-deficient mice.  |  Hashida, T., et al. 2002. Endocrinology. 143: 2808-11. PMID: 12072416
4. L63, the Drosophila PFTAIRE, interacts with two novel proteins unrelated to cyclins.  |  Rascle, A., et al. 2003. Mech Dev. 120: 617-28. PMID: 12782278
5. An RNA interference-based screen identifies MAP4K4/NIK as a negative regulator of PPARgamma, adipogenesis, and insulin-responsive hexose transport.  |  Tang, X., et al. 2006. Proc Natl Acad Sci U S A. 103: 2087-92. PMID: 16461467
6. A Cdc2-related protein kinase hPFTAIRE1 from human brain interacting with 14-3-3 proteins.  |  Gao, Y., et al. 2006. Cell Res. 16: 539-47. PMID: 16775625
7. A novel cdc2-related protein kinase expressed in the nervous system.  |  Lazzaro, MA., et al. 1997. J Neurochem. 69: 348-64. PMID: 9202329
8. Chromosomal mapping of the PFTAIRE gene, Pftk1, a cdc2-related kinase expressed predominantly in the mouse nervous system.  |  Lazzaro, MA. and Julien, JP. 1997. Genomics. 42: 536-7. PMID: 9205131
9. The identification and characterization of expression of Pftaire-1, a novel Cdk family member, suggest its function in the mouse testis and nervous system.  |  Besset, V., et al. 1998. Mol Reprod Dev. 50: 18-29. PMID: 9547506

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PFTAIRE-1 Antibody (C-3) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-PFTAIRE-1 Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PFTAIRE-1 Antibody (C-3) Specifications

Alternate Product Name: PFTAIRE-1

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-140

Epitope Target: PFTAIRE-1

Epitope Species: human

Product Reactivity/Detection: PFTAIRE-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for PFTAIRE-1 Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent