R2 Antibody (A-5): sc-398294

R2 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect R2 protein, also known as RRM2, across mouse, rat, human, and additional species including canine. R2 monoclonal antibody (A-5) targets a precise epitope mapping to amino acids 30-56 near the N-terminus of human R2, ensuring accurate and reliable binding for various applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). R2 protein is a critical subunit of ribonucleotide reductase, an essential enzyme complex responsible for the synthesis and maintenance of deoxyribonucleoside triphosphates (dNTPs), which are vital for DNA replication and repair. R2 is localized in the cytoplasm and acts as the limiting factor for the catalytic activity of ribonucleotide reductase, with expression tightly correlated to the S-phase of the cell cycle. This regulation ensures a sufficient supply of dNTPs necessary for DNA synthesis, thereby maintaining genomic stability and enabling proper cell division. R2 interacts with the R1 subunit to form the active ribonucleotide reductase complex, facilitating efficient dNTP production during periods of rapid cell proliferation and in response to DNA damage. Anti-R2 antibody (A-5) supports advanced research into cell cycle regulation, DNA replication, and the molecular mechanisms underlying various diseases, including cancer. The broad species reactivity and high specificity make R2 (A-5) monoclonal antibody an indispensable tool for scientists investigating the functional dynamics of ribonucleotide reductase in diverse biological contexts, thereby contributing to a deeper understanding of cellular proliferation and DNA repair processes.

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R2 Antibody (A-5) References:

1. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage.  |  Tanaka, H., et al. 2000. Nature. 404: 42-9. PMID: 10716435
2. Controlled protein degradation regulates ribonucleotide reductase activity in proliferating mammalian cells during the normal cell cycle and in response to DNA damage and replication blocks.  |  Chabes, A. and Thelander, L. 2000. J Biol Chem. 275: 17747-53. PMID: 10747958
3. Sequence analysis of the large and small subunits of human ribonucleotide reductase.  |  Pavloff, N., et al. 1992. DNA Seq. 2: 227-34. PMID: 1627826
4. The involvement of Arg265 of mouse ribonucleotide reductase R2 protein in proton transfer and catalysis.  |  Narváez, AJ., et al. 2006. J Biol Chem. 281: 26022-8. PMID: 16829694
5. S-phase-specific expression of mammalian ribonucleotide reductase R1 and R2 subunit mRNAs.  |  Björklund, S., et al. 1990. Biochemistry. 29: 5452-8. PMID: 1696835
6. Excess ribonucleotide reductase R2 subunits coordinate the S phase checkpoint to facilitate DNA damage repair and recovery from replication stress.  |  Lin, ZP., et al. 2007. Biochem Pharmacol. 73: 760-72. PMID: 17188250
7. RNA interference targeting the R2 subunit of ribonucleotide reductase inhibits growth of tumor cells in vitro and in vivo.  |  Avolio, TM., et al. 2007. Anticancer Drugs. 18: 377-88. PMID: 17351390
8. Induction of the mouse ribonucleotide reductase R1 and R2 genes in response to DNA damage by UV light.  |  Filatov, D., et al. 1996. J Biol Chem. 271: 23698-704. PMID: 8798592
9. Two YY-1-binding proximal elements regulate the promoter strength of the TATA-less mouse ribonucleotide reductase R1 gene.  |  Johansson, E., et al. 1998. J Biol Chem. 273: 29816-21. PMID: 9792697

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-R2 Antibody (A-5) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-R2 Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

R2 Antibody (A-5) Specifications

Alternate Product Name: R2

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 30-56

Epitope Target: R2

Epitope Species: human

Product Reactivity/Detection: R2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for R2 Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin