AIRE-1 Antibody (C-2): sc-373703

AIRE-1 Antibody (C-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects AIRE-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). AIRE-1 (C-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AIRE-1 protein, also known as autoimmune regulator type 1, plays a crucial role in the immune system by regulating self-antigen expression in the thymus, which is essential for self-tolerance development and autoimmune disease prevention. AIRE-1 is localized in medullary epithelial cells and monocyte-dendritic cells of the thymus, where AIRE-1 orchestrates transcriptional control of genes necessary for proper thymic environment establishment. This localization ensures developing T cells are exposed to diverse self-antigens, allowing autoreactive T cell elimination during negative selection. Mutations in AIRE-1 can lead to impaired thymic T cell selection, contributing to autoimmune conditions like autoimmune polyendocrinopathy candidiasis ectodermal dystrophy (APECED). Using anti-AIRE-1 antibody (C-2) in research can provide insights into immune tolerance mechanisms and AIRE-1 dysfunction implications in autoimmune diseases.

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AIRE-1 Antibody (C-2) References:

1. Association analysis of the cytotoxic T lymphocyte antigen-4 (CTLA-4) and autoimmune regulator-1 (AIRE-1) genes in sporadic autoimmune Addison's disease.  |  Vaidya, B., et al. 2000. J Clin Endocrinol Metab. 85: 688-91. PMID: 10690877
2. Autoimmune hepatitis.  |  Obermayer-Straub, P., et al. 2000. J Hepatol. 32: 181-97. PMID: 10728804
3. Normal thymic architecture and negative selection are associated with Aire expression, the gene defective in the autoimmune-polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).  |  Zuklys, S., et al. 2000. J Immunol. 165: 1976-83. PMID: 10925280
4. Subcellular localization of the autoimmune regulator protein. characterization of nuclear targeting and transcriptional activation domain.  |  Pitkänen, J., et al. 2001. J Biol Chem. 276: 19597-602. PMID: 11274163
5. Identification of a novel mutation in the autoimmune regulator (AIRE-1) gene in a French family with autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy.  |  Saugier-Veber, P., et al. 2001. Eur J Endocrinol. 144: 347-51. PMID: 11275943
6. Screening for an AIRE-1 mutation in patients with Addison's disease, type 1 diabetes, Graves' disease and Hashimoto's thyroiditis as well as in APECED syndrome.  |  Meyer, G., et al. 2001. Clin Endocrinol (Oxf). 54: 335-8. PMID: 11298085
7. Natural history of asplenism in APECED--patient report.  |  Starzyk, J., et al. 2001. J Pediatr Endocrinol Metab. 14: 443-9. PMID: 11327379
8. Autoimmune regulator AIRE: evidence for genetic differences between autoimmune hepatitis and hepatitis as part of the autoimmune polyglandular syndrome type 1.  |  Vogel, A., et al. 2001. Hepatology. 33: 1047-52. PMID: 11343230
9. AIRE-1 (autoimmune regulator type 1) as a regulator of the thymic induction of negative selection.  |  Park, Y., et al. 2003. Ann N Y Acad Sci. 1005: 431-5. PMID: 14679106
10. Autoimmune regulator-1 messenger ribonucleic acid analysis in a novel intronic mutation and two additional novel AIRE gene mutations in a cohort of autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy patients.  |  Podkrajsek, KT., et al. 2005. J Clin Endocrinol Metab. 90: 4930-5. PMID: 15886230
11. Absence of Heterozygous K83E and R257X Mutations of the AIRE-1 Gene in 46 Children with Type 1 Diabetes and 44 Children with Graves' Disease.  |  Iwama, S., et al. 2005. Clin Pediatr Endocrinol. 14: 17-21. PMID: 24790305
12. A common and recurrent 13-bp deletion in the autoimmune regulator gene in British kindreds with autoimmune polyendocrinopathy type 1.  |  Pearce, SH., et al. 1998. Am J Hum Genet. 63: 1675-84. PMID: 9837820

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AIRE-1 Antibody (C-2) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-AIRE-1 Antibody (C-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AIRE-1 Antibody (C-2) Specifications

Alternate Product Name: AIRE

Product Clone Name: C-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 246-545

Epitope Target: AIRE-1

Epitope Species: human

Product Reactivity/Detection: AIRE-1 isoforms 1, 2 and 3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for AIRE-1 Antibody (C-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin