BAF Antibody (A-11): sc-166324
- BAF Antibody (A-11) is a mouse monoclonal IgG2a κ BAF antibody, cited in 11 publications, provided at 200 µg/ml
- raised against amino acids 1-89 representing full length barrier-to-autointegration factor of human origin
- BAF Antibody (A-11) is recommended for detection of BAF of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and bovine
- Anti-BAF Antibody (A-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-166324 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of BAF (A-11): sc-166324.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BAF Antibody (A-11) for WB applications. These reagents are now offered in bundles with BAF Antibody (A-11) (see ordering information below).
BAF Antibody (A-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects BAF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-BAF antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Barrier-to-autointegration factor (BAF) plays a critical role in the nucleus, where BAF is primarily located, as BAF binds non-specifically to double-stranded DNA and is essential for maintaining chromatin structure. This localization is vital because BAF facilitates the recruitment of nuclear proteins through interaction with the LEM domain, which is crucial for chromatin decondensation and membrane recruitment during nuclear assembly. Furthermore, BAF′s ability to compress chromatin structure is significant for regulating gene expression in a tissue- or cell type-specific manner, as BAF interacts with various homeodomain transcription factors. Notably, retroviruses such as HIV-1 exploit BAF from host cells, incorporating BAF into preintegration complexes to prevent autointegration of retroviral DNA, thereby enhancing the integration of viral DNA into the host chromosome.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BAF Antibody (A-11) References:
- Distinct functional domains in emerin bind lamin A and DNA-bridging protein BAF. | Lee, KK., et al. 2001. J Cell Sci. 114: 4567-73. PMID: 11792821
- BAF is required for emerin assembly into the reforming nuclear envelope. | Haraguchi, T., et al. 2001. J Cell Sci. 114: 4575-85. PMID: 11792822
- Identification of the autoantigen HB as the barrier-to-autointegration factor. | Forné, I., et al. 2003. J Biol Chem. 278: 50641-4. PMID: 14523012
- Barrier-to-autointegration factor BAF binds p55 Gag and matrix and is a host component of human immunodeficiency virus type 1 virions. | Mansharamani, M., et al. 2003. J Virol. 77: 13084-92. PMID: 14645565
- BAF complex vulnerabilities in cancer demonstrated via structure-based PROTAC design. | Farnaby, W., et al. 2019. Nat Chem Biol. 15: 672-680. PMID: 31178587
- Structure of nucleosome-bound human BAF complex. | He, S., et al. 2020. Science. 367: 875-881. PMID: 32001526
- A Structural Model of the Endogenous Human BAF Complex Informs Disease Mechanisms. | Mashtalir, N., et al. 2020. Cell. 183: 802-817.e24. PMID: 33053319
- The BAF chromatin remodeling complexes: structure, function, and synthetic lethalities. | Varga, J., et al. 2021. Biochem Soc Trans. 49: 1489-1503. PMID: 34431497
- BAF Complex Maintains Glioma Stem Cells in Pediatric H3K27M Glioma. | Panditharatna, E., et al. 2022. Cancer Discov. 12: 2880-2905. PMID: 36305736
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BAF Antibody (A-11) | sc-166324 | 200 µg/ml | $316.00 | |||
BAF Antibody (A-11): m-IgG Fc BP-HRP Bundle | sc-528943 | 200 µg Ab; 10 µg BP | $354.00 | |||
BAF Antibody (A-11): m-IgGκ BP-HRP Bundle | sc-521566 | 200 µg Ab, 40 µg BP | $354.00 | |||
BAF Antibody (A-11): m-IgG2a BP-HRP Bundle | sc-547212 | 200 µg Ab; 10 µg BP | $354.00 | |||
BAF Antibody (A-11) X | sc-166324 X | 200 µg/0.1 ml | $316.00 | |||
BAF Antibody (A-11) AC | sc-166324 AC | 500 µg/ml, 25% agarose | $416.00 | |||
BAF Antibody (A-11) HRP | sc-166324 HRP | 200 µg/ml | $316.00 | |||
BAF Antibody (A-11) FITC | sc-166324 FITC | 200 µg/ml | $330.00 | |||
BAF Antibody (A-11) PE | sc-166324 PE | 200 µg/ml | $343.00 | |||
BAF Antibody (A-11) Alexa Fluor® 488 | sc-166324 AF488 | 200 µg/ml | $357.00 | |||
BAF Antibody (A-11) Alexa Fluor® 546 | sc-166324 AF546 | 200 µg/ml | $357.00 | |||
BAF Antibody (A-11) Alexa Fluor® 594 | sc-166324 AF594 | 200 µg/ml | $357.00 | |||
BAF Antibody (A-11) Alexa Fluor® 647 | sc-166324 AF647 | 200 µg/ml | $357.00 | |||
BAF Antibody (A-11) Alexa Fluor® 680 | sc-166324 AF680 | 200 µg/ml | $357.00 | |||
BAF Antibody (A-11) Alexa Fluor® 790 | sc-166324 AF790 | 200 µg/ml | $357.00 |