BLM Antibody (C-1): sc-376237

BLM Antibody (C-1) is a mouse monoclonal IgG1 antibody that detects BLM in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). BLM, encoded by the BLM gene, plays a crucial role in maintaining genomic stability by unwinding DNA during replication and repair processes, which is vital for preventing mutations that can lead to cancer. BLM is primarily located in the nucleus, where BLM interacts with both single- and double-stranded DNA, and BLM′s ATPase activity is essential for helicase function. Notably, BLM′s ability to unwind G-quadruplex (G4) DNA structures is significant, as these structures can impede replication and transcription if not properly resolved. Dysfunction of BLM, often due to mutations in the BLM gene, leads to an increased frequency of sister chromatid exchanges, a hallmark of Bloom′s syndrome, which is associated with a higher risk of developing various cancers. BLM Antibody (C-1) enables researchers to gain insights into the mechanisms of DNA repair and the implications of BLM dysfunction in cancer biology.

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BLM Antibody (C-1) References:

1. Transfection of BLM into cultured bloom syndrome cells reduces the sister-chromatid exchange rate toward normal.  |  Ellis, NA., et al. 1999. Am J Hum Genet. 65: 1368-74. PMID: 10521302
2. The function of Xenopus Bloom's syndrome protein homolog (xBLM) in DNA replication.  |  Liao, S., et al. 2000. Genes Dev. 14: 2570-5. PMID: 11040210
3. Functional link between BLM defective in Bloom's syndrome and the ataxia-telangiectasia-mutated protein, ATM.  |  Beamish, H., et al. 2002. J Biol Chem. 277: 30515-23. PMID: 12034743
4. Protein kinase A subunit expression is altered in Bloom syndrome fibroblasts and the BLM protein is increased in adrenocortical hyperplasias: inverse findings for BLM and PRKAR1A.  |  Heyerdahl, SL., et al. 2008. Horm Metab Res. 40: 391-7. PMID: 18401830
5. Non-Bloom syndrome-associated partial and total loss-of-function variants of BLM helicase.  |  Mirzaei, H. and Schmidt, KH. 2012. Proc Natl Acad Sci U S A. 109: 19357-62. PMID: 23129629
6. Nonsense mutation p.Q548X in BLM, the gene mutated in Bloom's syndrome, is associated with breast cancer in Slavic populations.  |  Prokofyeva, D., et al. 2013. Breast Cancer Res Treat. 137: 533-9. PMID: 23225144
7. Blm-s, a BH3-only protein enriched in postmitotic immature neurons, is transcriptionally upregulated by p53 during DNA damage.  |  Liu, WW., et al. 2014. Cell Rep. 9: 166-179. PMID: 25263558
8. Discovery of Isaindigotone Derivatives as Novel Bloom's Syndrome Protein (BLM) Helicase Inhibitors That Disrupt the BLM/DNA Interactions and Regulate the Homologous Recombination Repair.  |  Yin, QK., et al. 2019. J Med Chem. 62: 3147-3162. PMID: 30827110
9. BLM helicase protein negatively regulates stress granule formation through unwinding RNA G-quadruplex structures.  |  Danino, YM., et al. 2023. Nucleic Acids Res. 51: 9369-9384. PMID: 37503837
10. Point mutations causing Bloom's syndrome abolish ATPase and DNA helicase activities of the BLM protein.  |  Bahr, A., et al. 1998. Oncogene. 17: 2565-71. PMID: 9840919

The preferred BLM antibody is BLM Antibody (B-4): sc-365753.