Myc Antibody (G-4): sc-373712
- Myc Antibody (G-4) is a mouse monoclonal IgG1 κ, cited in 17 publications, provided at 200 µg/ml
- raised against amino acids 1-262 of c-Myc of human origin
- Anti-Myc Antibody (G-4) is recommended for detection of c-Myc p67 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Myc Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-373712 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Myc (G-4): sc-373712.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Myc Antibody (G-4) for WB applications. These reagents are now offered in bundles with Myc Antibody (G-4) (see ordering information below).
c-Myc Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 1-262 of human c-Myc, a critical transcription factor that regulates numerous cellular processes, including cell proliferation, differentiation, and metabolism. c-Myc mouse monoclonal antibody (G-4) recognizes an epitope within the c-Myc immunogen sequence and demonstrates effectiveness in Western blotting, immunoprecipitation, immunofluorescence, and ELISA applications. Anti-c-Myc monoclonal antibody (G-4) serves as an invaluable tool for studying the c-Myc/Max interaction and downstream transcriptional programs. c-Myc exerts biological functions largely through interaction with Max protein, a relationship essential for c-Myc′s activity. The c-Myc/Max interaction occurs through their basic helix-loop-helix leucine zipper (bHLH-Zip) domains, which facilitate heterodimer formation. Once dimerized, the c-Myc/Max complex binds to specific DNA sequences, known as E-box elements, regulating the transcription of genes involved in cell cycle progression, metabolism, and cellular growth. The significance of the c-Myc/Max interaction lies in the fact that neither protein can efficiently bind DNA or regulate transcription independently. Max, though capable of dimerizing with other bHLH-Zip proteins such as Mad1 or Mxi1, requires c-Myc to activate transcription. In contrast, c-Myc alone has low affinity for DNA and requires Max to form a stable, functional complex. The c-Myc/Max heterodimer proves essential for promoting the transcription of genes that drive cellular growth and proliferation, making c-Myc a key player in normal cellular function. However, dysregulation of this interaction, particularly through overexpression of c-Myc, is a hallmark of many cancers. c-Myc frequently undergoes overexpression or amplification in various tumors, including those of the lung, breast, and colon, leading to aberrant cell growth, resistance to apoptosis, and tumorigenesis. Additionally, the c-Myc/Max complex participates in fine-tuned regulation of cellular metabolism. c-Myc controls the expression of genes involved in glycolysis, mitochondrial function, and ribosome biogenesis, supporting the increased energy demands and biosynthetic needs of rapidly proliferating cells. This ability to modulate both cell growth and metabolism underscores the oncogenic potential of c-Myc when regulation becomes disrupted. c-Myc (G-4) antibody enables researchers to investigate the mechanisms by which c-Myc drives cellular transformation and cancer progression, as well as examine the c-Myc/Max interaction in oncogenic pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Myc Antibody (G-4) | sc-373712 | 200 µg/ml | $316.00 | |||
Myc Antibody (G-4): m-IgG Fc BP-HRP Bundle | sc-529543 | 200 µg Ab; 10 µg BP | $354.00 | |||
Myc Antibody (G-4): m-IgGκ BP-HRP Bundle | sc-522466 | 200 µg Ab, 40 µg BP | $354.00 | |||
Myc Antibody (G-4): m-IgG1 BP-HRP Bundle | sc-543569 | 200 µg Ab; 20 µg BP | $354.00 | |||
Myc Antibody (G-4) X | sc-373712 X | 200 µg/0.1 ml | $316.00 | |||
Myc Antibody (G-4) AC | sc-373712 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Myc Antibody (G-4) HRP | sc-373712 HRP | 200 µg/ml | $316.00 | |||
Myc Antibody (G-4) FITC | sc-373712 FITC | 200 µg/ml | $330.00 | |||
Myc Antibody (G-4) PE | sc-373712 PE | 200 µg/ml | $343.00 | |||
Myc Antibody (G-4) Alexa Fluor® 488 | sc-373712 AF488 | 200 µg/ml | $357.00 | |||
Myc Antibody (G-4) Alexa Fluor® 546 | sc-373712 AF546 | 200 µg/ml | $357.00 | |||
Myc Antibody (G-4) Alexa Fluor® 594 | sc-373712 AF594 | 200 µg/ml | $357.00 | |||
Myc Antibody (G-4) Alexa Fluor® 647 | sc-373712 AF647 | 200 µg/ml | $357.00 | |||
Myc Antibody (G-4) Alexa Fluor® 680 | sc-373712 AF680 | 200 µg/ml | $357.00 | |||
Myc Antibody (G-4) Alexa Fluor® 790 | sc-373712 AF790 | 200 µg/ml | $357.00 |