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E2F1 Antibody (KH95): sc-251

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Datasheets
  • E2F1 Antibody (KH95) is a mouse monoclonal IgG2a κ E2F1 antibody, cited in 769 publications, provided at 200 µg/ml
  • raised against amino acids 342-386 of E2F-1 of human origin
  • E2F1 Antibody (KH95) is recommended for detection of E2F-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • Anti-E2F1 Antibody (KH95) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • TransCruz reagent for Gel Supershift and ChIP applications (sc-251 X, 200 µg/0.1 ml)
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of E2F1 (KH95): sc-251.
  • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for E2F1 Antibody (KH95) for WB and IHC(P) applications. These reagents are now offered in bundles with E2F1 Antibody (KH95) (see ordering information below).

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E2F-1 Antibody (KH95) is a mouse monoclonal IgG2a kappa light chain antibody that specifically targets E2F-1, a key transcription factor involved in the regulation of cell cycle progression. E2F-1 monoclonal antibody (KH95) is raised against amino acids 342-386 of human E2F-1 and is reactive with E2F-1 from human, mouse, and rat sources, making it suitable for a variety of applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). E2F-1 is a member of the E2F family of transcription factors, which includes E2F-2, E2F-3, E2F-4, E2F-5, E2F-6, and E2F-7, all of which regulate genes critical for the G1 and S phases of the cell cycle. E2F-1 forms functional heterodimers with DP-1, and this complex drives the expression of genes that promote cell cycle progression and DNA replication. E2F-1 is particularly important in regulating the transition from the G1 phase to the S phase, and E2F-1 activity is tightly controlled by interactions with the retinoblastoma protein (Rb). Rb binding inhibits E2F-1 transcriptional activity, preventing the expression of cell cycle genes until appropriate signals trigger Rb inactivation, a process often disrupted in cancer. E2F-1 plays a critical role in both normal cell cycle control and in the dysregulation of cell proliferation seen in various malignancies. E2F-1 monoclonal antibody (KH95) is valuable for studies examining the role of E2F-1 in cell cycle regulation and its involvement in cancer research, as well as in understanding the broader E2F/Rb signaling network.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

E2F1 Antibody (KH95) References:

  1. pRb and E2f-1 in mouse development and tumorigenesis.  |  Macleod, K. 1999. Curr Opin Genet Dev. 9: 31-9. PMID: 10072353
  2. E2F-1 induced apoptosis.  |  Phillips, AC. and Vousden, KH. 2001. Apoptosis. 6: 173-82. PMID: 11388666
  3. A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F.  |  Helin, K., et al. 1992. Cell. 70: 337-50. PMID: 1638634
  4. E2F-1 induces melanoma cell apoptosis via PUMA up-regulation and Bax translocation.  |  Hao, H., et al. 2007. BMC Cancer. 7: 24. PMID: 17263886
  5. The E2F transcription factor is a cellular target for the RB protein.  |  Chellappan, SP., et al. 1991. Cell. 65: 1053-61. PMID: 1828392
  6. The T/E1A-binding domain of the retinoblastoma product can interact selectively with a sequence-specific DNA-binding protein.  |  Chittenden, T., et al. 1991. Cell. 65: 1073-82. PMID: 1828394
  7. E2F-1 overexpression inhibits human gastric cancer MGC-803 cell growth in vivo.  |  Wei, WY., et al. 2015. World J Gastroenterol. 21: 491-501. PMID: 25593464
  8. E2F-4, a new member of the E2F transcription factor family, interacts with p107.  |  Ginsberg, D., et al. 1994. Genes Dev. 8: 2665-79. PMID: 7958924
  9. E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo.  |  Beijersbergen, RL., et al. 1994. Genes Dev. 8: 2680-90. PMID: 7958925
  10. Binding to DNA and the retinoblastoma gene product promoted by complex formation of different E2F family members.  |  Krek, W., et al. 1993. Science. 262: 1557-60. PMID: 8248803
  11. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation.  |  Helin, K., et al. 1993. Genes Dev. 7: 1850-61. PMID: 8405995
  12. E2F-6, a member of the E2F family that can behave as a transcriptional repressor.  |  Trimarchi, JM., et al. 1998. Proc Natl Acad Sci U S A. 95: 2850-5. PMID: 9501179

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

E2F1 Antibody (KH95)

sc-251
200 µg/ml
$316.00

E2F1 Antibody (KH95): m-IgG Fc BP-HRP Bundle

sc-525421
200 µg Ab; 10 µg BP
$354.00

E2F1 Antibody (KH95): m-IgG2a BP-HRP Bundle

sc-546245
200 µg Ab; 10 µg BP
$354.00

E2F1 Antibody (KH95) X

sc-251 X
200 µg/0.1 ml
$316.00

E2F1 Antibody (KH95) AC

sc-251 AC
500 µg/ml, 25% agarose
$416.00

E2F1 Antibody (KH95) HRP

sc-251 HRP
200 µg/ml
$316.00

E2F1 Antibody (KH95) FITC

sc-251 FITC
200 µg/ml
$330.00

E2F1 Antibody (KH95) PE

sc-251 PE
200 µg/ml
$343.00

E2F1 Antibody (KH95) Alexa Fluor® 488

sc-251 AF488
200 µg/ml
$357.00

E2F1 Antibody (KH95) Alexa Fluor® 546

sc-251 AF546
200 µg/ml
$357.00

E2F1 Antibody (KH95) Alexa Fluor® 594

sc-251 AF594
200 µg/ml
$357.00

E2F1 Antibody (KH95) Alexa Fluor® 647

sc-251 AF647
200 µg/ml
$357.00

E2F1 Antibody (KH95) Alexa Fluor® 680

sc-251 AF680
200 µg/ml
$357.00

E2F1 Antibody (KH95) Alexa Fluor® 790

sc-251 AF790
200 µg/ml
$357.00

この抗体で組織免疫染色は可能ですか?ポジティブコントロールとしては乳がん組織、正常腎臓組織は使えますか? 2次抗体は何ですか?

Asked by: uchi
E2F1 Antibody (KH95) は、マウス、ラット、ヒト由来の E2F-1 の WB、IP、IF、IHC(P) による検出に推奨されています。組織には関わらず、マウス、ラット、ヒト由来のサンプルには使えます。WB、IHC実験に2次抗体はm-IgG Fc BP-HRP: sc-525409をお勧めします。宜しくお願い致します。 E2F1 Antibody (KH95) is recommended for WB, IP, IF, and IHC(P) detection of E2F-1 from mouse, rat, and human sources. It can be used for samples of mouse, rat, and human origin, regardless of the tissue. m-IgG Fc BP-HRP: sc-525409 is recommended as a secondary antibody for WB and IHC experiments. Thank you.
Answered by: Jiajun Z
Date published: 2024-05-16

I would like to use the E2F1 antibody (KH95) sc-251 for IHC. What protocol (retrieval solution, time etc.) would you recommend me for the HIER?

Asked by: RR04
Thank you for your question. You can find our recommended protocol for IHC staining (including antigen retrieval) under this link: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Support Europe
Date published: 2020-08-18

Is this Antibody good for ChIP?

Asked by: Katie214
Thank you for your question. Yes, this antibody is recommended and warrantied for IP use, which does include ChIP.
Answered by: Tech Service
Date published: 2019-02-08

Hi! I would like to try E2F-1 (KH95) sc-251 to test for IF in my cells before I order it. Can I get the 10 ug free sample?

Asked by: juliasero
Thank you for the question and your interest in our products. We will contact you by email.
Answered by: Technical Support Europe
Date published: 2018-02-02

What is the recommended secondary reagent to be used in Western blot?

Asked by: Anonymous
We recommend our Mouse IgG Binding Protein conjugated to HRP as a secondary detection reagent: https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 6
Date published: 2016-12-16
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Rated 5 out of 5 by from 免疫印迹结果先是申请了小样,然后购买了产品,免疫印迹结果检测A549和H1299细胞中E2F1的表达,1:600稀释,结果不错
Date published: 2025-02-15
Rated 5 out of 5 by from clear and strong signalUsed for WB and got a clear and strong signal at the expected molecular weight.
Date published: 2022-07-19
Rated 1 out of 5 by from Typical TrashLike every other SC antibody, this antibody is absolute trash!
Date published: 2020-07-03
Rated 4 out of 5 by from Good for WB and IF!I used this antibody in WB and IF in HeLa cells and it worked pretty good.
Date published: 2019-11-11
Rated 5 out of 5 by from It works great for western blot.SC-251 works great for western blot with HUVECs cell lines.
Date published: 2018-11-12
Rated 4 out of 5 by from good productIt is a good antibody. We got clean band but some background for WB.
Date published: 2018-09-11
Rated 3 out of 5 by from e2f1There was a band of weak signal with some non-specific bands in western blotting
Date published: 2018-04-18
Rated 2 out of 5 by from no good signal in IFI didn't detect good staining signal in 4%PFA fixed embyronic mouse brain(after antigen retrieval)
Date published: 2018-03-07
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E2F1 Antibody (KH95) is rated 4.6 out of 5 by 34.
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