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E2F-5 Antibody (H-1): sc-271497

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Datasheets
  • E2F-5 Antibody (H-1) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 89-200 of E2F-5 of human origin
  • recommended for detection of E2F-5 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and porcine; non cross-reactive with E2F-6
  • TransCruz reagent for ChIP application (sc-271497 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for E2F-5 Antibody (H-1) for WB applications. These reagents are now offered in bundles with E2F-5 Antibody (H-1) (see ordering information below).

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E2F-5 Antibody (H-1) is a mouse monoclonal IgG1 antibody that detects E2F-5 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). E2F-5 plays a crucial role in the regulation of the cell cycle, particularly in the transition from the G1 phase to the S phase, by forming heterodimers with the DP-1 protein to create an active E2F transcriptional regulatory complex. This complex is essential for the transcriptional activation of genes that are vital for DNA synthesis and cell proliferation. Dysregulation of E2F-5 can lead to uncontrolled cell growth and has been implicated in various cancers, making E2F-5 a significant target for therapeutic intervention. E2F-5′s interaction with other members of the E2F family, such as E2F-1, E2F-2, and E2F-3, further highlights E2F-5′s importance in maintaining proper cell cycle control and potential as a biomarker for tumor progression. Anti-E2F-5 antibody (H-1) is an invaluable tool for researchers studying cell cycle dynamics and the molecular mechanisms underlying cancer development.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

E2F-5 Antibody (H-1)

sc-271497
200 µg/ml
$316.00

E2F-5 Antibody (H-1): m-IgG Fc BP-HRP Bundle

sc-537647
200 µg Ab; 10 µg BP
$354.00

E2F-5 Antibody (H-1): m-IgGκ BP-HRP Bundle

sc-534980
200 µg Ab; 40 µg BP
$354.00

E2F-5 Antibody (H-1): m-IgG1 BP-HRP Bundle

sc-545281
200 µg Ab; 20 µg BP
$354.00

E2F-5 Antibody (H-1) X

sc-271497 X
200 µg/0.1 ml
$316.00

What blocking solution should I use with E2F-5 (H-1): sc-271497 monoclonal antibody for Western blot?

Asked by: jerojero
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
  • y_2025, m_4, d_11, h_6CST
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Rated 4 out of 5 by from Good band in WB in AGood band in WB in A-673 and A-431 nuclear extracts. -SCBT QC
Date published: 2015-05-09
Rated 5 out of 5 by from Strong band in WB in mouse E2FStrong band in WB in mouse E2F-5 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-29
Rated 5 out of 5 by from Published data for WB inPublished data for WB in
Date published: 2015-02-06
Rated 5 out of 5 by from Good specificity in WB in KNRK whole cell lysateGood specificity in WB in KNRK whole cell lysate. -SCBT QC
Date published: 2014-06-06
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E2F-5 Antibody (H-1) is rated 4.8 out of 5 by 4.
  • y_2025, m_4, d_11, h_6
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