EGR1 Antibody (S-25): sc-101033

Egr-1 Antibody (S-25) is a mouse monoclonal IgG2a kappa light chain antibody that detects Egr-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Egr-1 antibody (S-25) is available in a non-conjugated form, allowing for versatile applications in various experimental settings. Egr-1, a member of the early growth response (Egr) family of transcription factors, plays a crucial role in cellular processes such as mitogenesis and differentiation by binding to specific DNA sequences, including the Egr-site (5′-CGCCCCCGC-3′). This binding activates the transcription of target genes essential for cell growth and development. Rapid response to extracellular stimuli underscores Egr-1′s importance in cellular signaling pathways, particularly in response to stress and growth factors. Various post-translational modifications, including phosphorylation and glycosylation, can influence Egr-1′s activity and stability, thereby affecting gene expression patterns. Understanding the dynamics of Egr-1 and its regulatory mechanisms is vital for elucidating its role in physiological and pathological processes, including cancer and neurodegenerative diseases.

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EGR1 Antibody (S-25) References:

1. Egr-1 is activated by 17beta-estradiol in MCF-7 cells by mitogen-activated protein kinase-dependent phosphorylation of ELK-1.  |  Chen, CC., et al. 2004. J Cell Biochem. 93: 1063-74. PMID: 15449318
2. Stretch-inducible expression of the angiogenic factor CCN1 in vascular smooth muscle cells is mediated by Egr-1.  |  Grote, K., et al. 2004. J Biol Chem. 279: 55675-81. PMID: 15492009
3. HGF induces fibronectin matrix synthesis in melanoma cells through MAP kinase-dependent signaling pathway and induction of Egr-1.  |  Gaggioli, C., et al. 2005. Oncogene. 24: 1423-33. PMID: 15608673
4. Essential interaction of Egr-1 at an islet-specific response element for basal and gastrin-dependent glucagon gene transactivation in pancreatic alpha-cells.  |  Leung-Theung-Long, S., et al. 2005. J Biol Chem. 280: 7976-84. PMID: 15611055
5. The MAPK pathway and Egr-1 mediate stress-related behavioral effects of glucocorticoids.  |  Revest, JM., et al. 2005. Nat Neurosci. 8: 664-72. PMID: 15834420
6. Egr transcription factors in the nervous system.  |  Beckmann, AM. and Wilce, PA. 1997. Neurochem Int. 31: 477-510; discussion 517-6. PMID: 9307998
7. The human zinc finger protein EGR-4 acts as autoregulatory transcriptional repressor.  |  Zipfel, PF., et al. 1997. Biochim Biophys Acta. 1354: 134-44. PMID: 9396630

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EGR1 Antibody (S-25) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-EGR1 Antibody (S-25) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EGR1 Antibody (S-25) Specifications

Alternate Product Name: Egr-1

Product Clone Name: S-25

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 444-544

Epitope Target: Egr-1

Epitope Species: human

Product Reactivity/Detection: Egr-1 p82

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for EGR1 Antibody (S-25): N/A