HIF1a Antibody (3C144): sc-71247

HIF-1α Antibody (3C144) is a mouse monoclonal IgG1 kappa light chain antibody engineered to target the hypoxia-inducible factor 1-alpha (HIF-1α) protein, recognizing an epitope within amino acids 329-530 of human HIF-1α. anti-HIF-1α antibody (3C144) is indispensable for researchers investigating the structural and functional dynamics of HIF-1α, a pivotal transcription factor that orchestrates cellular responses to oxygen deprivation. HIF-1α, also called HIF1, MOP1, PASD8, and HIF-1alpha, is characterized by its multi-domain structure, including the basic helix-loop-helix (bHLH) domain essential for DNA binding, the PAS (Per-Arnt-Sim) domains that facilitate dimerization with HIF-1β (Arnt1), and the oxygen-dependent degradation (ODD) domain that regulates stability under normoxic conditions. HIF-1α contains transactivation domains crucial for recruiting co-activators and initiating transcription of target genes. Understanding the intricate structure of HIF-1α is critical, as this determines the ability to form the active HIF-1 complex, bind to hypoxia-responsive elements (HRE) in the genome, and activate transcription of genes involved in essential processes such as glycolysis, erythropoiesis, and angiogenesis, which are vital for restoring oxygen homeostasis and maintaining cell viability under hypoxic stress. HIF-1α monoclonal antibody (3C144) exhibits broad species reactivity, effectively detecting HIF-1α in mouse, rat, and human samples, as well as in additional species including bovine and porcine, across various applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). anti-HIF-1α antibody (3C144) is available in both conjugated and non-conjugated forms including conjugates with horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants. By enabling precise detection and analysis of HIF-1α′s structural domains, anti-HIF-1α antibody (3C144) supports critical research into the molecular mechanisms underlying hypoxia responses and their implications in various physiological and pathological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HIF1a Antibody (3C144) References:

1. Dynamic HIF1A regulation during human placental development.  |  Ietta, F., et al. 2006. Biol Reprod. 75: 112-21. PMID: 16611863
2. HIF1A is essential for the development of the intermediate plexus of the retinal vasculature.  |  Caprara, C., et al. 2011. Invest Ophthalmol Vis Sci. 52: 2109-17. PMID: 21212189
3. The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability.  |  Bett, JS., et al. 2013. Biochem J. 451: 185-94. PMID: 23398456
4. HIF1A and EPAS1 mRNA and protein expression during in vitro culture of human primary term cytotrophoblasts and effect of oxygen tension on their expression.  |  Depoix, CL., et al. 2016. Reprod Biol. 16: 203-211. PMID: 27692362
5. HIF1A and EPAS1 potentiate hypoxia-induced upregulation of inhibin alpha chain expression in human term cytotrophoblasts in vitro.  |  Depoix, CL., et al. 2017. Mol Hum Reprod. 23: 199-209. PMID: 28115494
6. Hypoxia-induced shedding of MICA and HIF1A-mediated immune escape of pancreatic cancer cells from NK cells: role of circ_0000977/miR-153 axis.  |  Ou, ZL., et al. 2019. RNA Biol. 16: 1592-1603. PMID: 31402756
7. Loss of HIF1A From Pancreatic Cancer Cells Increases Expression of PPP1R1B and Degradation of p53 to Promote Invasion and Metastasis.  |  Tiwari, A., et al. 2020. Gastroenterology. 159: 1882-1897.e5. PMID: 32768595
8. HIF1A Alleviates compression-induced apoptosis of nucleus pulposus derived stem cells via upregulating autophagy.  |  He, R., et al. 2021. Autophagy. 17: 3338-3360. PMID: 33455530
9. CXCR4, regulated by HIF1A, promotes endometrial breakdown via CD45+ leukocyte recruitment in a mouse model of menstruation.  |  Wang, S., et al. 2023. Reprod Biol. 23: 100785. PMID: 37392490
10. USP51 facilitates colorectal cancer stemness and chemoresistance by forming a positive feed-forward loop with HIF1A.  |  Mu, M., et al. 2023. Cell Death Differ. 30: 2393-2407. PMID: 37816999

The preferred HIF1a antibody is HIF1a Antibody (H1alpha 67): sc-53546.