HP1γ Antibody (F-1): sc-398562
- HP1γ Antibody (F-1) is a mouse monoclonal IgG2a κ HP1γ antibody, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 1-104 mapping at the N-terminus of HP1γ of human origin
- HP1 gamma Antibody (F-1) is recommended for detection of HP1γ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-HP1 gamma Antibody (F-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HP1γ (F-1): sc-398562.
- m-IgG2a BP-HRP is the preferred secondary detection reagent for HP1γ Antibody (F-1) for WB and IHC(P) applications. This reagent is now offered in a bundle with HP1γ Antibody (F-1) (see ordering information below).
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HP1γ Antibody (F-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects HP1 gamma protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-HP1γ antibody (F-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HP1 gamma protein plays a crucial role in forming and maintaining heterochromatin, which is essential for regulating gene expression and maintaining genomic stability. Heterochromatin is characterized by a densely packed structure, which limits access to transcription machinery, thereby silencing gene expression in those regions. HP1γ interaction with chromatin is particularly important during the cell cycle, associating with newly synthesized histones and heterochromatin proteins during DNA replication, ensuring proper chromatin assembly. HP1γ is involved in chromatin remodeling dynamics, reassociating with chromatin at the end of mitosis, coinciding with Histone H3 dephosphorylation. This regulation is vital for maintaining chromosomal structural integrity and preventing genomic instability, which can lead to various diseases, including cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HP1γ Antibody (F-1) References:
- Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells. | Minc, E., et al. 1999. Chromosoma. 108: 220-34. PMID: 10460410
- Heterochromatin dynamics in mouse cells: interaction between chromatin assembly factor 1 and HP1 proteins. | Murzina, N., et al. 1999. Mol Cell. 4: 529-40. PMID: 10549285
- Duplication and maintenance of heterochromatin domains. | Taddei, A., et al. 1999. J Cell Biol. 147: 1153-66. PMID: 10601331
- Identification of heterochromatin protein 1 (HP1) as a phosphorylation target by Pim-1 kinase and the effect of phosphorylation on the transcriptional repression function of HP1(1). | Koike, N., et al. 2000. FEBS Lett. 467: 17-21. PMID: 10664448
- Purification and characterization of CAF-I, a human cell factor required for chromatin assembly during DNA replication in vitro. | Smith, S. and Stillman, B. 1989. Cell. 58: 15-25. PMID: 2546672
- The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication. | Kaufman, PD., et al. 1995. Cell. 81: 1105-14. PMID: 7600578
- Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4. | Verreault, A., et al. 1996. Cell. 87: 95-104. PMID: 8858152
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HP1γ Antibody (F-1) | sc-398562 | 200 µg/ml | $316.00 | |||
HP1γ Antibody (F-1): m-IgG2a BP-HRP Bundle | sc-545973 | 200 µg Ab; 10 µg BP | $354.00 | |||
HP1γ Antibody (F-1) AC | sc-398562 AC | 500 µg/ml, 25% agarose | $416.00 | |||
HP1γ Antibody (F-1) HRP | sc-398562 HRP | 200 µg/ml | $316.00 | |||
HP1γ Antibody (F-1) FITC | sc-398562 FITC | 200 µg/ml | $330.00 | |||
HP1γ Antibody (F-1) PE | sc-398562 PE | 200 µg/ml | $343.00 | |||
HP1γ Antibody (F-1) Alexa Fluor® 488 | sc-398562 AF488 | 200 µg/ml | $357.00 | |||
HP1γ Antibody (F-1) Alexa Fluor® 546 | sc-398562 AF546 | 200 µg/ml | $357.00 | |||
HP1γ Antibody (F-1) Alexa Fluor® 594 | sc-398562 AF594 | 200 µg/ml | $357.00 | |||
HP1γ Antibody (F-1) Alexa Fluor® 647 | sc-398562 AF647 | 200 µg/ml | $357.00 | |||
HP1γ Antibody (F-1) Alexa Fluor® 680 | sc-398562 AF680 | 200 µg/ml | $357.00 | |||
HP1γ Antibody (F-1) Alexa Fluor® 790 | sc-398562 AF790 | 200 µg/ml | $357.00 |