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p-NFKBIA/IkB alpha Antibody (39A1431): sc-52943

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Datasheets
  • p-NFKBIA/IkB alpha Antibody (39A1431) is a mouse monoclonal IgG1 κ, cited in 25 publications, provided at 50 µg/0.5 ml
  • raised against synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of p-IκB-α of human origin
  • recommended for detection of Ser 32 and Ser 36 phosphorylated IκB-α of mouse, rat and human origin by WB
  • See p-NFKBIA/IkB alpha (B-9): sc-8404 for p-NFKBIA/IkB alpha antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-NFKBIA/IkB alpha Antibody (39A1431). This work is in progress.

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p-IκB-α Antibody (39A1431) is a mouse monoclonal IgG1 antibody that detects p-IκB-α in mouse, rat, and human samples through western blotting (WB). p-IκB-α plays a crucial role in regulating the NF-κB signaling pathway, which controls various cellular processes, including inflammation, immune response, and cell survival. Phosphorylation of IκB-α leads to inhibitor degradation, allowing NF-κB dimers to translocate to the nucleus and activate target genes. This post-translational modification enables timely and appropriate responses to cellular stress and inflammatory signals, making p-IκB-α a key player in maintaining cellular homeostasis. p-IκB-α (39A1431) monoclonal antibody provides reliable detection of phosphorylated IκB-α, enabling researchers to study its role in various biological contexts and disease states, particularly those related to dysregulated NF-κB activity.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

p-NFKBIA/IkB alpha Antibody (39A1431)

sc-52943
50 µg/0.5 ml
$316.00

How much sample do you recommend loading on the gel for Western blot detection using p-IκB-α (39A1431): sc-52943?

Asked by: Dr Ninau Qelp
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from Published with this antibody in SNU601 BARF1Published with this antibody in SNU601 BARF1 cells for western blot analysis. -SCBT Publication Review
Date published: 2013-05-10
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p-NFKBIA/IkB alpha Antibody (39A1431) is rated 4.0 out of 5 by 1.
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