MAGOH Antibody (C-11): sc-271405

MAGOH Antibody (C-11) is a mouse monoclonal IgG2b antibody that detects MAGOH in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAGOH plays a vital role in embryo development and is ubiquitously expressed across various adult tissues, highlighting its importance in cellular functions. MAGOH features a unique structure characterized by an extremely flat, six-stranded anti-parallel β sheet adjacent to two helices, which is crucial for interaction with the Y14 protein. This interaction forms a complex that is essential for post-splicing processing, including the nuclear export and cytoplasmic localization of mRNA, as well as the surveillance process known as nonsense-mediated mRNA decay (NMD). The MAGOH-Y14 complex remains associated with mRNA even after export to the cytoplasm, necessitating translation of mRNA for eventual removal. This structural and functional relationship underscores MAGOH′s significance in maintaining mRNA integrity and regulating gene expression, making anti-MAGOH antibody (C-11) an invaluable tool for researchers studying these critical biological processes.

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MAGOH Antibody (C-11) References:

1. The exon-junction complex proteins, Y14 and MAGOH regulate STAT3 activation.  |  Muromoto, R., et al. 2009. Biochem Biophys Res Commun. 382: 63-8. PMID: 19254694
2. The exon junction complex component Magoh controls brain size by regulating neural stem cell division.  |  Silver, DL., et al. 2010. Nat Neurosci. 13: 551-8. PMID: 20364144
3. Genetic analyses using a mouse cell cycle mutant identifies magoh as a novel gene involved in Cdk regulation.  |  Inaki, M., et al. 2011. Genes Cells. 16: 166-78. PMID: 21210908
4. Phosphorylation status of human RNA-binding protein 8A in cells and its inhibitory regulation by Magoh.  |  Ishigaki, Y., et al. 2015. Exp Biol Med (Maywood). 240: 438-45. PMID: 25349214
5. Dosage-dependent requirements of Magoh for cortical interneuron generation and survival.  |  Sheehan, CJ., et al. 2020. Development. 147: PMID: 31857347
6. Development of Magoh protein-overexpressing HEK cells for optimized therapeutic protein production.  |  Mufarrege, EF., et al. 2021. Biotechnol Appl Biochem. 68: 230-238. PMID: 32249976
7. The EJC component Magoh in non-vertebrate chordates.  |  Sepe, RM., et al. 2020. Dev Genes Evol. 230: 295-304. PMID: 32632492
8. Multifaceted roles of MAGOH Proteins.  |  Mitra, R., et al. 2023. Mol Biol Rep. 50: 1931-1941. PMID: 36396768
9. MAGOH is correlated with poor prognosis and is essential for cell proliferation in lower-grade glioma.  |  Xiao, F., et al. 2023. Aging (Albany NY). 15: 5713-5733. PMID: 37390121
10. MAGOH promotes gastric cancer progression via hnRNPA1 expression inhibition-mediated RONΔ160/PI3K/AKT signaling pathway activation.  |  Yu, S., et al. 2024. J Exp Clin Cancer Res. 43: 32. PMID: 38268030

The preferred MAGOH antibody is MAGOH Antibody (21B12): sc-56724.