PUS1 Antibody (C-4): sc-376442

PUS1 Antibody (C-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects PUS1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-PUS1 antibody (C-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PUS1, or pseudouridine synthase 1, is a crucial enzyme that catalyzes the conversion of uridine to pseudouridine in RNA, a modification that enhances the stability and functionality of tRNA and rRNA molecules. This post-translational modification is vital for proper protein synthesis and cellular function, as pseudouridine improves the structural integrity of RNA, thereby influencing the efficiency of translation and overall fidelity of gene expression. PUS1 is predominantly located in the nucleus, where PUS1 forms complexes with other proteins, such as retinoic acid receptor gamma (RARG) and SRA1 RNA, underscoring PUS1′s role in RNA processing and regulation. PUS1 expression is widespread, with particularly high levels found in brain and skeletal muscle tissues, and mutations in the PUS1 gene are associated with myopathy with lactic acidosis and sideroblastic anemia (MLASA), a rare genetic disorder affecting oxidative phosphorylation in muscle and bone marrow. The deduced human PUS1 protein consists of 348 amino acids and exhibits 92% sequence homology with its mouse counterpart, highlighting the evolutionary conservation of PUS1′s function across species.

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PUS1 Antibody (C-4) References:

1. Cloning and characterization of a mammalian pseudouridine synthase.  |  Chen, J. and Patton, JR. 1999. RNA. 5: 409-19. PMID: 10094309
2. Pseudouridine synthetase Pus1 of Saccharomyces cerevisiae: kinetic characterisation, tRNA structural requirement and real-time analysis of its complex with tRNA.  |  Arluison, V., et al. 1999. J Mol Biol. 289: 491-502. PMID: 10356324
3. RNA:pseudouridine synthetase Pus1 from Saccharomyces cerevisiae: oligomerization property and stoichiometry of the complex with yeast tRNA(Phe).  |  Arluison, V., et al. 1999. Biochimie. 81: 751-6. PMID: 10492022
4. Mouse pseudouridine synthase 1: gene structure and alternative splicing of pre-mRNA.  |  Chen, J. and Patton, JR. 2000. Biochem J. 352 Pt 2: 465-73. PMID: 11085940
5. Caenorhabditis elegans pseudouridine synthase 1 activity in vivo: tRNA is a substrate, but not U2 small nuclear RNA.  |  Patton, JR. and Padgett, RW. 2003. Biochem J. 372: 595-602. PMID: 12597772
6. Missense mutation in pseudouridine synthase 1 (PUS1) causes mitochondrial myopathy and sideroblastic anemia (MLASA).  |  Bykhovskaya, Y., et al. 2004. Am J Hum Genet. 74: 1303-8. PMID: 15108122
7. Mitochondrial myopathy and sideroblastic anemia (MLASA): missense mutation in the pseudouridine synthase 1 (PUS1) gene is associated with the loss of tRNA pseudouridylation.  |  Patton, JR., et al. 2005. J Biol Chem. 280: 19823-8. PMID: 15772074
8. Transfer RNA-pseudouridine synthetase Pus1 of Saccharomyces cerevisiae contains one atom of zinc essential for its native conformation and tRNA recognition.  |  Arluison, V., et al. 1998. Biochemistry. 37: 7268-76. PMID: 9585540

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PUS1 Antibody (C-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PUS1 Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PUS1 Antibody (C-4) Specifications

Alternate Product Name: PUS1

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 148-427

Epitope Target: PUS1

Epitope Species: human

Product Reactivity/Detection: PUS1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PUS1 Antibody (C-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin