p-Pol II Antibody (8A7): sc-13583
- p-Pol II Antibody (8A7) is a mouse monoclonal IgM κ, cited in 26 publications, provided at 200 µg/ml
- raised against a synthetic peptide containing phosphorylated Ser 1801 of p-Pol II of human origin
- recommended for detection of Ser 1801 phosphorylated RNA polymerase II of human origin and correspondingly phosphorylated RNA polymerase II of mouse, rat and S. cerevisiae origin by WB, IP, IF and IHC(P)
- TransCruz reagent for Gel Supershift and ChIP applications (sc-13583 X, 200 µg/0.1 ml)
- m-IgGκ BP-HRP is the preferred secondary detection reagent for p-Pol II Antibody (8A7) for WB and IHC(P) applications. This reagent is now offered in a bundle with p-Pol II Antibody (8A7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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p-Pol II Antibody (8A7) is a mouse monoclonal IgM antibody that detects RNA polymerase II (Pol II) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Pol II antibody (8A7) plays a crucial role in studying the function of RNA polymerase II, a multi-subunit enzyme essential for the transcription of protein-coding genes. RNA polymerase II is responsible for synthesizing messenger RNA from a DNA template, a process vital for gene expression and regulation. Proper functioning of RNA polymerase II ensures accurate transcription of genes into RNA, which is then translated into proteins that perform various functions within the cell. Dysregulation of RNA polymerase II activity can lead to a range of diseases, including cancer and genetic disorders, making RNA polymerase II an important target for research. Pol II monoclonal antibody (8A7)′s ability to recognize and bind to specific phosphorylated forms of RNA polymerase II allows researchers to investigate enzymatic activity and regulatory mechanisms in different biological contexts, providing valuable insights into cellular processes and disease mechanisms.
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p-Pol II Antibody (8A7) References:
- Phosphorylated RNA polymerase II stimulates pre-mRNA splicing. | Hirose, Y., et al. 1999. Genes Dev. 13: 1234-9. PMID: 10346811
- Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms. | Bushnell, DA., et al. 2004. Science. 303: 983-8. PMID: 14963322
- Structure and function of RNA polymerase II. | Cramer, P. 2004. Adv Protein Chem. 67: 1-42. PMID: 14969722
- Mechanism of RNA polymerase I transcription. | Comai, L. 2004. Adv Protein Chem. 67: 123-55. PMID: 14969726
- The RNA polymerase II transcription cycle: cycling through chromatin. | Svejstrup, JQ. 2004. Biochim Biophys Acta. 1677: 64-73. PMID: 15020047
- RNA polymerase III transcription and cancer. | White, RJ. 2004. Oncogene. 23: 3208-16. PMID: 15094770
- BRCA1 cooperates with NUFIP and P-TEFb to activate transcription by RNA polymerase II. | Cabart, P., et al. 2004. Oncogene. 23: 5316-29. PMID: 15107825
- Epidermal growth factor enhances cellular TATA binding protein levels and induces RNA polymerase I- and III-dependent gene activity. | Zhong, S., et al. 2004. Mol Cell Biol. 24: 5119-29. PMID: 15169879
- Distinct mechanisms for repression of RNA polymerase III transcription by the retinoblastoma tumor suppressor protein. | Hirsch, HA., et al. 2004. Mol Cell Biol. 24: 5989-99. PMID: 15199152
- Downstream DNA selectively affects a paused conformation of human RNA polymerase II. | Palangat, M., et al. 2004. J Mol Biol. 341: 429-42. PMID: 15276834
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Pol II Antibody (8A7) | sc-13583 | 200 µg/ml | $316.00 | |||
p-Pol II Antibody (8A7): m-IgGκ BP-HRP Bundle | sc-551922 | 200 µg Ab; 40 µg BP | $354.00 | |||
p-Pol II Antibody (8A7) X | sc-13583 X | 200 µg/0.1 ml | $316.00 |