p-SC35 Antibody (SC-35): sc-53518

p-SC35 Antibody (SC-35) is a mouse monoclonal IgG1 kappa light chain antibody that detects p-SC35 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-p-SC35 antibody (SC-35) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p-SC35 plays a crucial role in pre-mRNA splicing by acting as a splicing enhancer, which is essential for accurate recognition of splice sites and regulation of alternative splicing. This function is vital because alternative splicing allows a single gene to produce multiple protein isoforms, thereby increasing protein diversity from the genome. p-SC35 is primarily located in the nucleus, where p-SC35 interacts with other serine/arginine-rich splicing factors, such as 9G8 and SF2/ASF, to facilitate splicing. Nuclear localization of p-SC35 ensures splicing occurs close to transcription machinery, enhancing mRNA processing efficiency. Additionally, p-SC35 activity and other splicing factors are finely regulated by various proteins, including members of the heterogeneous nuclear ribonucleoprotein (hnRNP) family, which can modulate splicing outcomes by competing for binding sites on pre-mRNA. This intricate balance of interactions underscores p-SC35′s importance in gene expression regulation and potential implications in diseases associated with splicing dysregulation.

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p-SC35 Antibody (SC-35) References:

1. The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and phosphorylation.  |  Petersen-Mahrt, SK., et al. 1999. EMBO J. 18: 1014-24. PMID: 10022843
2. Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences.  |  Schaal, TD. and Maniatis, T. 1999. Mol Cell Biol. 19: 1705-19. PMID: 10022858
3. The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.  |  Cavaloc, Y., et al. 1999. RNA. 5: 468-83. PMID: 10094314
4. pPKCδ activates SC35 splicing factor during H9c2 myoblastic differentiation.  |  Zara, S., et al. 2011. Histol Histopathol. 26: 59-69. PMID: 21117027
5. Cyclic AMP-dependent protein kinase enhances SC35-promoted Tau exon 10 inclusion.  |  Chen, C., et al. 2014. Mol Neurobiol. 49: 615-24. PMID: 24037441
6. HIV-1-Tat Protein Inhibits SC35-mediated Tau Exon 10 Inclusion through Up-regulation of DYRK1A Kinase.  |  Kadri, F., et al. 2015. J Biol Chem. 290: 30931-46. PMID: 26534959
7. SIRT1 Deacetylates SC35 and Suppresses Its Function in Tau Exon 10 Inclusion.  |  Yin, X., et al. 2018. J Alzheimers Dis. 61: 561-570. PMID: 29226865
8. The nuclear GSK-3β regulated post-transcriptional processing of mRNA through phosphorylation of SC35.  |  An, Y., et al. 2019. Mol Cell Biochem. 451: 55-67. PMID: 30030778
9. Function of conserved domains of hnRNP A1 and other hnRNP A/B proteins.  |  Mayeda, A., et al. 1994. EMBO J. 13: 5483-95. PMID: 7957114
10. Specific commitment of different pre-mRNAs to splicing by single SR proteins.  |  Fu, XD. 1993. Nature. 365: 82-5. PMID: 8361546
11. The splicing factor SRp20 modifies splicing of its own mRNA and ASF/SF2 antagonizes this regulation.  |  Jumaa, H. and Nielsen, PJ. 1997. EMBO J. 16: 5077-85. PMID: 9305649
12. A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm.  |  Cáceres, JF., et al. 1998. Genes Dev. 12: 55-66. PMID: 9420331

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-SC35 Antibody (SC-35) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-p-SC35 Antibody (SC-35) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-SC35 Antibody (SC-35) Specifications

Alternate Product Name: SC35

Product Clone Name: SC-35

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: phosphorylated SC35

Epitope Target: SC35

Epitope Species: mammalian

Product Reactivity/Detection: a phospho-epitope of factor SC35

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Drosophila, Xenopus

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for p-SC35 Antibody (SC-35): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin