TCF7L2/TCF4 Antibody (D-4): sc-166699

TCF-4 Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects TCF-4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TCF-4 (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TCF-4 plays a crucial role in regulating gene expression, particularly in the Wnt signaling pathway, which is vital for cellular processes such as proliferation, differentiation, and survival. TCF-4 is primarily located in the cytoplasm, where TCF-4 forms a complex with β-catenin before translocating to the nucleus. This nuclear translocation is essential for activating Wnt target genes, many of which are associated with cancer progression. Additionally, TCF-4 interacts with c-Jun, and the phosphorylation-dependent interaction between c-Jun and TCF-4 is significant in regulating intestinal tumorigenesis, highlighting TCF-4′s importance in both developmental biology and cancer research. Furthermore, TCF-4 has been implicated in neuropsychiatric disorders, including bipolar affective disorder, underscoring TCF-4′s relevance in both health and disease.

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TCF7L2/TCF4 Antibody (D-4) References:

1. Tcf-1-mediated transcription in T lymphocytes: differential role for glycogen synthase kinase-3 in fibroblasts and T cells.  |  Staal, FJ., et al. 1999. Int Immunol. 11: 317-23. PMID: 10221643
2. The human T cell transcription factor-1 gene. Structure, localization, and promoter characterization.  |  van de Wetering, M., et al. 1992. J Biol Chem. 267: 8530-6. PMID: 1569101
3. Human immunodeficiency virus-restricted replication in astrocytes and the ability of gamma interferon to modulate this restriction are regulated by a downstream effector of the Wnt signaling pathway.  |  Carroll-Anzinger, D., et al. 2007. J Virol. 81: 5864-71. PMID: 17392368
4. Identification and cloning of TCF-1, a T lymphocyte-specific transcription factor containing a sequence-specific HMG box.  |  van de Wetering, M., et al. 1991. EMBO J. 10: 123-32. PMID: 1989880
5. TCF-4 Regulated lncRNA-XIST Promotes M2 Polarization Of Macrophages And Is Associated With Lung Cancer.  |  Sun, Y. and Xu, J. 2019. Onco Targets Ther. 12: 8055-8062. PMID: 31632059
6. miR-26b-5p/TCF-4 Controls the Adipogenic Differentiation of Human Adipose-derived Mesenchymal Stem Cells.  |  Luo, Y., et al. 2020. Cell Transplant. 29: 963689720934418. PMID: 32579400
7. VE-Cadherin modulates β-catenin/TCF-4 to enhance Vasculogenic Mimicry.  |  Delgado-Bellido, D., et al. 2023. Cell Death Dis. 14: 135. PMID: 36797281
8. An HMG-box-containing T-cell factor required for thymocyte differentiation.  |  Verbeek, S., et al. 1995. Nature. 374: 70-4. PMID: 7870176
9. Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC.  |  Morin, PJ., et al. 1997. Science. 275: 1787-90. PMID: 9065402
10. Wnt-1 induces growth, cytosolic beta-catenin, and Tcf/Lef transcriptional activation in Rat-1 fibroblasts.  |  Young, CS., et al. 1998. Mol Cell Biol. 18: 2474-85. PMID: 9566868
11. Control of neural crest cell fate by the Wnt signalling pathway.  |  Dorsky, RI., et al. 1998. Nature. 396: 370-3. PMID: 9845073
12. Restricted high level expression of Tcf-4 protein in intestinal and mammary gland epithelium.  |  Barker, N., et al. 1999. Am J Pathol. 154: 29-35. PMID: 9916915

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TCF7L2/TCF4 Antibody (D-4) has 52 citations in a variety of scientific publications.

Primary antibodies like Anti-TCF7L2/TCF4 Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TCF7L2/TCF4 Antibody (D-4) Specifications

Alternate Product Name: TCF-4

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 565-592

Epitope Target: TCF-4

Epitope Species: human

Product Reactivity/Detection: TCF-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 52 citations in various scientific publications

Available Conjugates/Forms for TCF7L2/TCF4 Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent