TFIIH p80 Antibody (G-2): sc-271206

TFIIH p80 Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects TFIIH p80 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-TFIIH p80 antibody (G-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TFIIH p80, also known as XPD or ERCC2, plays a crucial role in transcription and DNA repair mechanisms, functioning as a part of the TFIIH complex, which is essential for unwinding DNA during transcription initiation and nucleotide excision repair. TFIIH p80′s ATP-dependent helicase activity is vital for separating DNA strands, allowing RNA polymerase II to access the template strand for transcription. Mutations in the XPD gene have been linked to various disorders, including xeroderma pigmentosum and Cockayne syndrome, highlighting TFIIH p80′s significance in maintaining genomic stability and cellular integrity. TFIIH p80 (G-2) monoclonal antibody enables researchers to effectively study transcription regulation dynamics and cellular response to DNA damage, providing insights into fundamental biological processes and potential therapeutic targets.

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TFIIH p80 Antibody (G-2) References:

1. Cloning of the 62-kilodalton component of basic transcription factor BTF2.  |  Fischer, L., et al. 1992. Science. 257: 1392-5. PMID: 1529339
2. Factors involved in specific transcription by mammalian RNA polymerase II. Identification and characterization of factor IIH.  |  Flores, O., et al. 1992. J Biol Chem. 267: 2786-93. PMID: 1733973
3. Purification and interaction properties of the human RNA polymerase B(II) general transcription factor BTF2.  |  Gerard, M., et al. 1991. J Biol Chem. 266: 20940-5. PMID: 1939143
4. A presumed DNA helicase encoded by ERCC-3 is involved in the human repair disorders xeroderma pigmentosum and Cockayne's syndrome.  |  Weeda, G., et al. 1990. Cell. 62: 777-91. PMID: 2167179
5. ERCC2: cDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3.  |  Weber, CA., et al. 1990. EMBO J. 9: 1437-47. PMID: 2184031
6. An RNA polymerase II transcription factor has an associated DNA-dependent ATPase (dATPase) activity strongly stimulated by the TATA region of promoters.  |  Conaway, RC. and Conaway, JW. 1989. Proc Natl Acad Sci U S A. 86: 7356-60. PMID: 2552440
7. XPD localizes in mitochondria and protects the mitochondrial genome from oxidative DNA damage.  |  Liu, J., et al. 2015. Nucleic Acids Res. 43: 5476-88. PMID: 25969448
8. DNA translocation mechanism of an XPD family helicase.  |  Cheng, K. and Wigley, DB. 2018. Elife. 7: PMID: 30520735
9. Correlations of polymorphisms in XPD and XRCC1 with glaucoma.  |  Liu, D. 2020. Minerva Med. 111: 283-285. PMID: 31124634
10. Mechanism of lesion verification by the human XPD helicase in nucleotide excision repair.  |  Fu, I., et al. 2022. Nucleic Acids Res. 50: 6837-6853. PMID: 35713557
11. p44 and p34 subunits of the BTF2/TFIIH transcription factor have homologies with SSL1, a yeast protein involved in DNA repair.  |  Humbert, S., et al. 1994. EMBO J. 13: 2393-8. PMID: 8194529
12. Cloning and characterization of p52, the fifth subunit of the core of the transcription/DNA repair factor TFIIH.  |  Marinoni, JC., et al. 1997. EMBO J. 16: 1093-102. PMID: 9118947

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TFIIH p80 Antibody (G-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TFIIH p80 Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TFIIH p80 Antibody (G-2) Specifications

Alternate Product Name: TFIIH p80

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 611-760

Epitope Target: TFIIH p80

Epitope Species: human

Product Reactivity/Detection: TFIIH p80

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TFIIH p80 Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent