TFIIIB90-1/2/3/5 Antibody (A-8): sc-390821

TFIIIB90-1/2/3/5 Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects TFIIIB90 isoforms 1, 2, 3, and 5 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-TFIIIB90-1/2/3/5 antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TFIIIB90 plays a crucial role in the transcription of genes by RNA polymerase III, which synthesizes essential small RNAs such as tRNA and 5S rRNA. This function matters because proper regulation of RNA polymerase III activity drives cellular growth and proliferation, especially in rapidly dividing cells. Overexpression of TFIIIB90 and associated complexes, such as TFIIIC2, commonly occurs in transformed cell lines and various tumors, including ovarian carcinomas, suggesting a connection between TFIIIB90 activity and cancer progression. TFIIIC2 complex, which includes multiple subunits, recruits RNA polymerase III to promoter elements, enabling transcription of genes necessary for cell survival and growth. In many malignancies, inactivation of tumor suppressors like retinoblastoma (RB) and p53 disrupts regulatory mechanisms that normally control TFIIIB90 function, contributing to aberrant expression of genes promoting tumorigenesis.

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TFIIIB90-1/2/3/5 Antibody (A-8) References:

1. The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone-specific acetyltransferase activity.  |  Hsieh, YJ., et al. 1999. Mol Cell Biol. 19: 7697-704. PMID: 10523658
2. Interactions between the tetratricopeptide repeat-containing transcription factor TFIIIC131 and its ligand, TFIIIB70. Evidence for a conformational change in the complex.  |  Moir, RD., et al. 2000. J Biol Chem. 275: 26591-8. PMID: 10859316
3. Alternatively spliced hBRF variants function at different RNA polymerase III promoters.  |  McCulloch, V., et al. 2000. EMBO J. 19: 4134-43. PMID: 10921893
4. Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters.  |  Schramm, L., et al. 2000. Genes Dev. 14: 2650-63. PMID: 11040218
5. RNA polymerase III transcription factor TFIIIC2 is overexpressed in ovarian tumors.  |  Winter, AG., et al. 2000. Proc Natl Acad Sci U S A. 97: 12619-24. PMID: 11058163
6. Retinoblastoma protein disrupts interactions required for RNA polymerase III transcription.  |  Sutcliffe, JE., et al. 2000. Mol Cell Biol. 20: 9192-202. PMID: 11094071
7. RNA polymerase III transcription: its control by tumor suppressors and its deregulation by transforming agents.  |  Brown, TR., et al. 2000. Gene Expr. 9: 15-28. PMID: 11097422
8. Activation of mouse genes in transformed cells.  |  Scott, MR., et al. 1983. Cell. 34: 557-67. PMID: 6311432
9. Expression of neural BC1 RNA: induction in murine tumours.  |  Chen, W., et al. 1997. Eur J Cancer. 33: 288-92. PMID: 9135502

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TFIIIB90-1/2/3/5 Antibody (A-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-TFIIIB90-1/2/3/5 Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TFIIIB90-1/2/3/5 Antibody (A-8) Specifications

Alternate Product Name: TFIIIB90-1/2/3/5

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 251-290

Epitope Target: TFIIIB90 -1

Epitope Species: human

Product Reactivity/Detection: TFIIIB90 isoforms 1, 2, 3 and 5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for TFIIIB90-1/2/3/5 Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent