Estrogen Receptor alpha Antibody (10H12B10): sc-130072

Estrogen Receptor alpha Antibody (10H12B10) is a mouse monoclonal IgG1 kappa light chain engineered to detect human Estrogen Receptor alpha (ERα) in western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA) assays. Anti-ERα antibody (10H12B10) is raised against a recombinant protein corresponding to amino acids 301-595 of human ERα, targeting a critical region within the ligand-binding domain that is essential for the receptor′s function as a ligand-activated transcription factor involved in regulating the normal activities of reproductive tissues. ERα (10H12B10) monoclonal antibody recognizes a protein subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitination, which play pivotal roles in modulating activity, stability, and interactions with co-regulatory proteins and DNA. Phosphorylation of ERα can enhance or inhibit transcriptional activity and alter responsiveness to estrogen, while ubiquitination typically governs receptor degradation and turnover, thereby maintaining cellular homeostasis. ERα monoclonal antibody (10H12B10) supports comprehensive investigations into the distinct regulatory mechanisms of ERα and ERβ, their differential activation by various ligands, and their crucial roles in cellular signaling pathways and gene expression regulation.

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Estrogen Receptor alpha Antibody (10H12B10) References:

1. Changes in estrogenic regulation of estrogen receptor alpha mRNA and progesterone receptor mRNA in the female rat hypothalamus during aging: an in situ hybridization study.  |  Funabashi, T., et al. 2000. Neurosci Res. 38: 85-92. PMID: 10997581
2. Estrogen receptor alpha has a functional role in the mouse rete testis and efferent ductules.  |  Lee, KH., et al. 2000. Biol Reprod. 63: 1873-80. PMID: 11090460
3. Regulation of estrogen receptor alpha and progesterone receptor (isoform A and B) expression in cultured human endometrial cells.  |  Prange-Kiel, J., et al. 2001. Exp Clin Endocrinol Diabetes. 109: 231-7. PMID: 11453036
4. Simulation of the different biological activities of diethylstilbestrol (DES) on estrogen receptor alpha and estrogen-related receptor gamma.  |  Nam, K., et al. 2003. Biopolymers. 68: 130-8. PMID: 12579585
5. Immunolocalization of estrogen receptor beta in the mouse brain: comparison with estrogen receptor alpha.  |  Mitra, SW., et al. 2003. Endocrinology. 144: 2055-67. PMID: 12697714
6. Effect of estradiol on estrogen receptor-alpha gene expression and activity can be modulated by the ErbB2/PI 3-K/Akt pathway.  |  Stoica, GE., et al. 2003. Oncogene. 22: 7998-8011. PMID: 12970748
7. p130Cas interacts with estrogen receptor alpha and modulates non-genomic estrogen signaling in breast cancer cells.  |  Cabodi, S., et al. 2004. J Cell Sci. 117: 1603-11. PMID: 15020686
8. Non-classical regulation of estrogen receptor-alpha by ICI182,780.  |  Wang, MM., et al. 2004. J Steroid Biochem Mol Biol. 92: 51-62. PMID: 15544930
9. A variant of estrogen receptor-{alpha}, hER-{alpha}36: transduction of estrogen- and antiestrogen-dependent membrane-initiated mitogenic signaling.  |  Wang, Z., et al. 2006. Proc Natl Acad Sci U S A. 103: 9063-8. PMID: 16754886
10. Ablation of estrogen receptor alpha or beta eliminates sex differences in mechanical pain threshold in normal and inflamed mice.  |  Li, L., et al. 2009. Pain. 143: 37-40. PMID: 19285805

The preferred Estrogen Receptor alpha antibody is Estrogen Receptor alpha Antibody (F-10): sc-8002.