ACP1 Antibody (D-3): sc-390190
- ACP1 Antibody (D-3) is a mouse monoclonal IgG2b κ ACP1 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 8-156 mapping near the N-terminus of ACP1 of human origin
- ACP1 Antibody (D-3) is recommended for detection of ACP1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ACP1 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ACP1 (D-3): sc-390190.
- m-IgG2b BP-HRP is the preferred secondary detection reagent for ACP1 Antibody (D-3) for WB applications. This reagent is now offered in a bundle with ACP1 Antibody (D-3) (see ordering information below).
ACP1 Antibody (D-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects ACP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ACP1 (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ACP1, also known as red cell acid phosphatase, plays a crucial role in cellular metabolism by catalyzing the transfer of phosphate from phosphate ester substrates to suitable acceptor alcohols, such as methanol and glycerol. This enzymatic activity is vital for maintaining proper intracellular concentrations of flavoenzymes and flavin coenzymes, which are essential for various biochemical processes and overall cell homeostasis. The human ACP1 gene, located on chromosome 2p25, encodes two isozymes, Bf (alpha) and Bs (beta), which are both 157 amino acids long but differ in an internal 34-residue segment, leading to variations in substrate specificity. Notably, both isoforms are not glycosylated, which may influence their functional roles in different cellular contexts. Understanding the distinct functions of these isoforms can provide insights into their contributions to metabolic pathways and potential implications in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ACP1 Antibody (D-3) References:
- Sequencing, cloning, and expression of human red cell-type acid phosphatase, a cytoplasmic phosphotyrosyl protein phosphatase. | Wo, YY., et al. 1992. J Biol Chem. 267: 10856-65. PMID: 1587862
- Human red cell acid phosphatase (ACP1): the primary structure of the two pairs of isozymes encoded by the ACP1*A and ACP1*C alleles. | Dissing, J. and Johnsen, AH. 1992. Biochim Biophys Acta. 1121: 261-8. PMID: 1627603
- Human red cell acid phosphatase (ACP1). The amino acid sequence of the two isozymes Bf and Bs encoded by the ACP1*B allele. | Dissing, J., et al. 1991. J Biol Chem. 266: 20619-25. PMID: 1939112
- Phenotypic variation in the phosphotransferase activity of human red cell acid phosphatase (ACP1). | Golden, VL. and Sensabaugh, GF. 1986. Hum Genet. 72: 340-3. PMID: 3009301
- Human red cell acid phosphatase (ACP1): evidence for differences in the primary structure of the two isozymes encoded by the ACP1*B allele. | Dissing, J. and Sensabaugh, GF. 1987. Biochem Genet. 25: 919-27. PMID: 3450278
- Exon structure at the human ACP1 locus supports alternative splicing model for f and s isozyme generation. | Lazaruk, KD., et al. 1993. Biochem Biophys Res Commun. 196: 440-6. PMID: 8216326
- Gene structure, sequence, and chromosomal localization of the human red cell-type low-molecular-weight acid phosphotyrosyl phosphatase gene, ACP1. | Bryson, GL., et al. 1995. Genomics. 30: 133-40. PMID: 8586411
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACP1 Antibody (D-3) | sc-390190 | 200 µg/ml | $316.00 | |||
ACP1 Antibody (D-3): m-IgG2b BP-HRP Bundle | sc-548774 | 200 µg Ab; 10 µg BP | $354.00 | |||
ACP1 Antibody (D-3) AC | sc-390190 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ACP1 Antibody (D-3) HRP | sc-390190 HRP | 200 µg/ml | $316.00 | |||
ACP1 Antibody (D-3) FITC | sc-390190 FITC | 200 µg/ml | $330.00 | |||
ACP1 Antibody (D-3) PE | sc-390190 PE | 200 µg/ml | $343.00 | |||
ACP1 Antibody (D-3) Alexa Fluor® 488 | sc-390190 AF488 | 200 µg/ml | $357.00 | |||
ACP1 Antibody (D-3) Alexa Fluor® 546 | sc-390190 AF546 | 200 µg/ml | $357.00 | |||
ACP1 Antibody (D-3) Alexa Fluor® 594 | sc-390190 AF594 | 200 µg/ml | $357.00 | |||
ACP1 Antibody (D-3) Alexa Fluor® 647 | sc-390190 AF647 | 200 µg/ml | $357.00 | |||
ACP1 Antibody (D-3) Alexa Fluor® 680 | sc-390190 AF680 | 200 µg/ml | $357.00 | |||
ACP1 Antibody (D-3) Alexa Fluor® 790 | sc-390190 AF790 | 200 µg/ml | $357.00 |