Src Antibody (B-12): sc-8056
- Src Antibody (B-12) is a mouse monoclonal IgG2a κ Src antibody, cited in 273 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 513-537 at the C-terminus of c-Src of human origin
- Src Antibody (B-12) is recommended for detection of c-Src, Yes p62, Fyn p59, c-Fgr p55 and c-Src-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Src Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Src (B-12): sc-8056.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Src Antibody (B-12) for WB and IHC(P) applications. These reagents are now offered in bundles with Src Antibody (B-12) (see ordering information below).
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SEE ALSO...
c-Src Antibody (B-12) is a mouse monoclonal IgG2a kappa light chain antibody raised against amino acids 513-537 at the C-terminus of human c-Src, a non-receptor tyrosine kinase crucial for cellular signal transduction. c-Src mouse monoclonal antibody (B-12) recognizes an epitope within the C-terminal region and demonstrates cross-reactivity with multiple Src family members, detecting c-Src, Yes p62, Fyn p59, c-Fgr p55, and c-Src-2 proteins in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. As a member of the Src family kinases, c-Src plays a central role in regulating fundamental cellular processes including proliferation, differentiation, motility, and survival. c-Src functions as a critical signal transducer, conveying messages from growth factor receptors, integrins, and other cell surface molecules to influence cell adhesion, migration, and cytoskeletal organization. When c-Src becomes aberrantly activated, the resulting dysregulation of these signaling pathways can contribute to cancer progression, promote metastasis, and stimulate angiogenesis, making c-Src an important focus in oncology research and therapeutic development. Anti-c-Src monoclonal antibody (B-12) serves as an invaluable tool for investigating c-Src′s functional mechanisms and protein interactions in both normal physiological and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Src Antibody (B-12) References:
- SRC family kinases mediate epithelial Na+ channel inhibition by endothelin. | Gilmore, ES., et al. 2001. J Biol Chem. 276: 42610-7. PMID: 11560932
- src-related tyrosine protein kinases as signaling components in hematopoietic cells. | Eiseman, E. and Bolen, JB. 1990. Cancer Cells. 2: 303-10. PMID: 1704247
- Expression and interactions of the Src family of tyrosine protein kinases in T lymphocytes. | Bolen, JB., et al. 1991. Adv Cancer Res. 57: 103-49. PMID: 1950702
- Neurones express high levels of a structurally modified, activated form of pp60c-src. | Brugge, JS., et al. Nature. 316: 554-7. PMID: 2412121
- Blood platelets express high levels of the pp60c-src-specific tyrosine kinase activity. | Golden, A., et al. 1986. Proc Natl Acad Sci U S A. 83: 852-6. PMID: 2419901
- Alterations in pp60c-src accompany differentiation of neurons from rat embryo striatum. | Cartwright, CA., et al. 1987. Mol Cell Biol. 7: 1830-40. PMID: 2439897
- Developmental expression of two forms of pp60c-src in mouse brain. | Wiestler, OD. and Walter, G. 1988. Mol Cell Biol. 8: 502-4. PMID: 2447488
- The PDGF receptor phosphorylates Tyr 138 in the c-Src SH3 domain in vivo reducing peptide ligand binding. | Broome, MA. and Hunter, T. 1997. Oncogene. 14: 17-34. PMID: 9010229
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Src Antibody (B-12) | sc-8056 | 200 µg/ml | $316.00 | |||
Src Antibody (B-12): m-IgG Fc BP-HRP Bundle | sc-528218 | 200 µg Ab; 10 µg BP | $354.00 | |||
Src Antibody (B-12): m-IgGκ BP-HRP Bundle | sc-520542 | 200 µg Ab, 40 µg BP | $354.00 | |||
Src Antibody (B-12): m-IgG2a BP-HRP Bundle | sc-547050 | 200 µg Ab; 10 µg BP | $354.00 | |||
Src Antibody (B-12) AC | sc-8056 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Src Antibody (B-12) HRP | sc-8056 HRP | 200 µg/ml | $316.00 | |||
Src Antibody (B-12) FITC | sc-8056 FITC | 200 µg/ml | $330.00 | |||
Src Antibody (B-12) PE | sc-8056 PE | 200 µg/ml | $343.00 | |||
Src Antibody (B-12) Alexa Fluor® 488 | sc-8056 AF488 | 200 µg/ml | $357.00 | |||
Src Antibody (B-12) Alexa Fluor® 546 | sc-8056 AF546 | 200 µg/ml | $357.00 | |||
Src Antibody (B-12) Alexa Fluor® 594 | sc-8056 AF594 | 200 µg/ml | $357.00 | |||
Src Antibody (B-12) Alexa Fluor® 647 | sc-8056 AF647 | 200 µg/ml | $357.00 | |||
Src Antibody (B-12) Alexa Fluor® 680 | sc-8056 AF680 | 200 µg/ml | $357.00 | |||
Src Antibody (B-12) Alexa Fluor® 790 | sc-8056 AF790 | 200 µg/ml | $357.00 | |||
Src (B-12) Neutralizing Peptide | sc-8056 P | 100 µg/0.5 ml | $68.00 |