casein kinase Iα Antibody (H-7): sc-74582

Casein kinase Iα Antibody (H-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects casein kinase I alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-casein kinase Iα antibody (H-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Casein kinase I alpha, also known as CSNK1A1L, is a 337 amino acid serine/threonine kinase that plays a crucial role in several cellular processes, including the regulation of the Wnt signaling pathway, which is vital for cell proliferation and differentiation. Casein kinase I alpha is primarily located in the cytoplasm, where casein kinase I alpha is involved in the phosphorylation of various substrates, thereby influencing key signaling pathways that govern cellular functions such as DNA repair, circadian rhythms, and apoptosis. Casein kinase I alpha′s ability to modulate these pathways underscores casein kinase I alpha′s importance in maintaining cellular homeostasis and potential implications in various diseases, including cancer and neurodegenerative disorders. With broad species reactivity and versatile applications, casein kinase Iα (H-7) antibody serves as an essential tool for researchers investigating the intricate roles of casein kinase I alpha in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

casein kinase Iα Antibody (H-7) References:

1. Hepatitis C virus NS5A is a direct substrate of casein kinase I-alpha, a cellular kinase identified by inhibitor affinity chromatography using specific NS5A hyperphosphorylation inhibitors.  |  Quintavalle, M., et al. 2007. J Biol Chem. 282: 5536-44. PMID: 17166835
2. Casein kinase I and II--multipotential serine protein kinases: structure, function, and regulation.  |  Tuazon, PT. and Traugh, JA. 1991. Adv Second Messenger Phosphoprotein Res. 23: 123-64. PMID: 1997039
3. Isolation and characterization of human cDNA clones encoding the alpha and the alpha' subunits of casein kinase II.  |  Lozeman, FJ., et al. 1990. Biochemistry. 29: 8436-47. PMID: 2174700
4. Involvement of hepatitis C virus NS5A hyperphosphorylation mediated by casein kinase I-α in infectious virus production.  |  Masaki, T., et al. 2014. J Virol. 88: 7541-55. PMID: 24760886
5. A phosphatidylinositol 4,5-bisphosphate-sensitive casein kinase I alpha associates with synaptic vesicles and phosphorylates a subset of vesicle proteins.  |  Gross, SD., et al. 1995. J Cell Biol. 130: 711-24. PMID: 7622570
6. Casein kinase I gamma subfamily. Molecular cloning, expression, and characterization of three mammalian isoforms and complementation of defects in the Saccharomyces cerevisiae YCK genes.  |  Zhai, L., et al. 1995. J Biol Chem. 270: 12717-24. PMID: 7759525
7. Isolation and characterization of human casein kinase I epsilon (CKI), a novel member of the CKI gene family.  |  Fish, KJ., et al. 1995. J Biol Chem. 270: 14875-83. PMID: 7797465
8. Protein kinases. 4. Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation.  |  Allende, JE. and Allende, CC. 1995. FASEB J. 9: 313-23. PMID: 7896000
9. Casein kinase II in signal transduction and cell cycle regulation.  |  Litchfield, DW. and Lüscher, B. 1993. Mol Cell Biochem. 127-128: 187-99. PMID: 7935350
10. Molecular cloning, expression, and characterization of a 49-kilodalton casein kinase I isoform from rat testis.  |  Graves, PR., et al. 1993. J Biol Chem. 268: 6394-401. PMID: 8454611
11. Casein kinase I alpha and alpha L: alternative splicing-generated kinases exhibit different catalytic properties.  |  Zhang, J., et al. 1996. Biochemistry. 35: 16319-27. PMID: 8973207
12. Tissue-specific distribution of mouse casein kinase I alpha mRNA.  |  McInnes, C. and Leader, DP. 1997. DNA Seq. 8: 55-7. PMID: 9522121

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-casein kinase Iα Antibody (H-7) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-casein kinase Iα Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

casein kinase Iα Antibody (H-7) Specifications

Alternate Product Name: CSNK1A1

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 281-337

Epitope Target: casein kinase Iα

Epitope Species: human

Product Reactivity/Detection: casein kinase Iα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for casein kinase Iα Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin