ERK 2 Antibody (D-2): sc-1647

ERK 2 Antibody (D-2) is a mouse monoclonal IgG2b kappa light chain antibody raised against amino acids 1-286 of ERK2 of human origin, specifically recognizing the p42 isoform of ERK2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin embedded sections (IHC/P), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). ERK2, also known as Mitogen-Activated Protein Kinase 1 (MAPK1), is a pivotal component of the MAPK/ERK signaling pathway, which transmits extracellular signals from growth factors, hormones, and neurotransmitters to elicit cellular responses. Activation of ERK2 occurs via phosphorylation by upstream kinases like MEK1/2, leading to dimerization and translocation into the nucleus. Once in the nucleus, ERK2 phosphorylates various transcription factors and regulatory proteins, influencing gene expression related to cell proliferation, differentiation, survival, and development. Dysregulation of ERK2 activity has been implicated in numerous pathological conditions, including cancer and neurodegenerative diseases, making ERK2 a significant focus in biomedical research. ERK 2 (D-2) antibody′s broad reactivity across species makes this product an invaluable tool for researchers investigating the critical roles of ERK2 in cell signaling and potential as a therapeutic target and research agent.

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ERK 2 Antibody (D-2) References:

1. Regulation of mitogen-activated protein kinase signaling networks by G protein-coupled receptors.  |  Gutkind, JS. 2000. Sci STKE. 2000: re1. PMID: 11752597
2. Fidelity and spatio-temporal control in MAP kinase (ERKs) signalling.  |  Pouysségur, J., et al. 2002. Biochem Pharmacol. 64: 755-63. PMID: 12213567
3. Extra-cellular signal-regulated ERK-1/ERK-2 pathway activation in human salivary gland mucoepidermoid carcinoma: association to aggressive tumor behavior and tumor cell proliferation.  |  Handra-Luca, A., et al. 2003. Am J Pathol. 163: 957-67. PMID: 12937136
4. ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ.  |  Haycock, JW., et al. 1992. Proc Natl Acad Sci U S A. 89: 2365-9. PMID: 1347949
5. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
6. Extracellular signal-regulated kinases in T cells: characterization of human ERK1 and ERK2 cDNAs.  |  Owaki, H., et al. 1992. Biochem Biophys Res Commun. 182: 1416-22. PMID: 1540184
7. ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.  |  Boulton, TG., et al. 1991. Cell. 65: 663-75. PMID: 2032290
8. Mechanism of host cell MAPK/ERK-2 incorporation into lentivirus particles: characterization of the interaction between MAPK/ERK-2 and proline-rich-domain containing capsid region of structural protein Gag.  |  Gupta, P., et al. 2011. J Mol Biol. 410: 681-97. PMID: 21762808
9. Tracking binding modes of 1,2,4-trisubstituted imidazolinone P38 MAP kinase and ERK-2 inhibitors.  |  Rao, SN. 2017. J Mol Graph Model. 76: 161-171. PMID: 28743070
10. Effect of Low-Intensity Pulsed Ultrasound Stimulation, Extracorporeal Shockwaves and Radial Pressure Waves on Akt, BMP-2, ERK-2, FAK and TGF-β1 During Bone Healing in Rat Tibial Defects.  |  Buarque de Gusmão, CV., et al. 2019. Ultrasound Med Biol. 45: 2140-2161. PMID: 31101448
11. Phosphorylation of the MAP kinase ERK2 promotes its homodimerization and nuclear translocation.  |  Khokhlatchev, AV., et al. 1998. Cell. 93: 605-15. PMID: 9604935
12. Sp1 phosphorylation by Erk 2 stimulates DNA binding.  |  Merchant, JL., et al. 1999. Biochem Biophys Res Commun. 254: 454-61. PMID: 9918860

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ERK 2 Antibody (D-2) has 615 citations in a variety of scientific publications.

Primary antibodies like Anti-ERK 2 Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ERK 2 Antibody (D-2) Specifications

Alternate Product Name: MAPK1

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 345-358

Epitope Target: ERK 2

Epitope Species: human

Product Reactivity/Detection: ERK 2 p42

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 615 citations in various scientific publications

Available Conjugates/Forms for ERK 2 Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred p-ERK antibody is p-ERK Antibody (E-4): sc-7383.