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IKK alpha Antibody (B-8): sc-7606

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Datasheets
  • IKK alpha Antibody (B-8) is a mouse monoclonal IgG2b κ IKK alpha antibody, cited in 100 publications, provided at 200 µg/ml
  • raised against amino acids 1-745 representing full length IKKα of human origin
  • IKK alpha Antibody (B-8) is recommended for detection of IKKα of human origin by WB, IP, IF, IHC(P), FCM and ELISA
  • Anti-IKK alpha Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
  • TransCruz reagent for ChIP application (sc-7606 X, 200 µg/0.1 ml)
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IKK alpha (B-8): sc-7606.
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IKK alpha Antibody (B-8) for WB and IHC(P) applications. These reagents are now offered in bundles with IKK alpha Antibody (B-8) (see ordering information below).

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IKKα Antibody (B-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects IKKα protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). IKKα Antibody (B-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IKKα plays a pivotal role in the regulation of the NFkB signaling pathway, which is crucial for controlling immune responses, inflammation, and cell survival. Located primarily in the cytoplasm, IKKα is part of a multi-subunit complex that includes IKKβ and IKKγ (NEMO), and is essential for the phosphorylation of the inhibitory protein IkB. This phosphorylation event is critical because IKKα leads to the degradation of IkB, allowing the transcription factor NFkB to translocate to the nucleus and activate target genes involved in inflammatory responses. IKKα interaction with IkB-α, particularly phosphorylation at serine residues 32 and 36, is a key regulatory step in the NFkB activation process, which is stimulated by upstream signals such as tumor necrosis factor (TNF) and interleukin-1 (IL-1) through the NFkB inducing kinase (NIK). Understanding IKKα function and regulation is vital for elucidating the mechanisms underlying various inflammatory diseases and developing targeted therapies.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

IKK alpha Antibody (B-8) References:

  1. Annexin-A1 SUMOylation regulates microglial polarization after cerebral ischemia by modulating IKKα stability via selective autophagy.  |  Li, X., et al. 2021. Sci Adv. 7: PMID: 33523920
  2. Targeting HECTD3-IKKα axis inhibits inflammation-related metastasis.  |  Li, F., et al. 2022. Signal Transduct Target Ther. 7: 264. PMID: 35918322
  3. The MGF300-2R protein of African swine fever virus is associated with viral pathogenicity by promoting the autophagic degradation of IKKα and IKKβ through the recruitment of TOLLIP.  |  Wang, T., et al. 2023. PLoS Pathog. 19: e1011580. PMID: 37566637
  4. Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation.  |  Verma, IM., et al. 1995. Genes Dev. 9: 2723-35. PMID: 7590248
  5. NF-kappa B: a lesson in family values.  |  Thanos, D. and Maniatis, T. 1995. Cell. 80: 529-32. PMID: 7867060
  6. CHUK, a new member of the helix-loop-helix and leucine zipper families of interacting proteins, contains a serine-threonine kinase catalytic domain.  |  Connelly, MA. and Marcu, KB. 1995. Cell Mol Biol Res. 41: 537-49. PMID: 8777433
  7. MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1.  |  Malinin, NL., et al. 1997. Nature. 385: 540-4. PMID: 9020361
  8. Identification and characterization of an IkappaB kinase.  |  Régnier, CH., et al. 1997. Cell. 90: 373-83. PMID: 9244310
  9. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB.  |  DiDonato, JA., et al. 1997. Nature. 388: 548-54. PMID: 9252186
  10. Tumor necrosis factor (TNF)-mediated kinase cascades: bifurcation of nuclear factor-kappaB and c-jun N-terminal kinase (JNK/SAPK) pathways at TNF receptor-associated factor 2.  |  Song, HY., et al. 1997. Proc Natl Acad Sci U S A. 94: 9792-6. PMID: 9275204
  11. The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation.  |  Zandi, E., et al. 1997. Cell. 91: 243-52. PMID: 9346241
  12. Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation.  |  Yamaoka, S., et al. 1998. Cell. 93: 1231-40. PMID: 9657155

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

IKK alpha Antibody (B-8)

sc-7606
200 µg/ml
$316.00

IKK alpha Antibody (B-8): m-IgG Fc BP-HRP Bundle

sc-528184
200 µg Ab; 10 µg BP
$354.00

IKK alpha Antibody (B-8): m-IgGκ BP-HRP Bundle

sc-520505
200 µg Ab, 40 µg BP
$354.00

IKK alpha Antibody (B-8): m-IgG2b BP-HRP Bundle

sc-550005
200 µg Ab; 10 µg BP
$354.00

IKK alpha Antibody (B-8) X

sc-7606 X
200 µg/0.1 ml
$316.00

IKK alpha Antibody (B-8) AC

sc-7606 AC
500 µg/ml, 25% agarose
$416.00

IKK alpha Antibody (B-8) HRP

sc-7606 HRP
200 µg/ml
$316.00

IKK alpha Antibody (B-8) FITC

sc-7606 FITC
200 µg/ml
$330.00

IKK alpha Antibody (B-8) PE

sc-7606 PE
200 µg/ml
$343.00

IKK alpha Antibody (B-8) Alexa Fluor® 488

sc-7606 AF488
200 µg/ml
$357.00

IKK alpha Antibody (B-8) Alexa Fluor® 546

sc-7606 AF546
200 µg/ml
$357.00

IKK alpha Antibody (B-8) Alexa Fluor® 594

sc-7606 AF594
200 µg/ml
$357.00

IKK alpha Antibody (B-8) Alexa Fluor® 647

sc-7606 AF647
200 µg/ml
$357.00

IKK alpha Antibody (B-8) Alexa Fluor® 680

sc-7606 AF680
200 µg/ml
$357.00

IKK alpha Antibody (B-8) Alexa Fluor® 790

sc-7606 AF790
200 µg/ml
$357.00

IKK alpha Antibody (B-8) TRITC

sc-7606 TRITC
200 µg/ml
$320.00

IKK alpha Antibody (B-8) Alexa Fluor® 405

sc-7606 AF405
200 µg/ml
$357.00

I found that IKKalpha has an upper band in my cells. In the IKKalpha knockout cells, the 85kDa band is absent, but this upper band still exists. I wondered if you had any idea what the reason was?

Asked by: ZoeZ
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-03-13

I would like to use this antibody to perform a double stain. Do you know how to do this with only mouse monoclonal primary antibodies?

Asked by: Cweed
We recommend using directly conjugated primary antibodies. This product, sc-7606, is available conjugated to FITC, AF488, and AF647.
Answered by: Technical Support 4
Date published: 2017-01-16
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Rated 5 out of 5 by from Great antibody-IKKαThis antibody worked great for me on western blot.
Date published: 2021-12-03
Rated 4 out of 5 by from WBGood Western Blot results of I KK-alpha expression in Huh7 cells,1:300 dilution was used.
Date published: 2018-09-15
Rated 4 out of 5 by from not very specific for WBthis antibody shows weak target band acompanies nonspecific bands in WB in human lung cencer cell lysates
Date published: 2017-08-02
Rated 5 out of 5 by from Very good antibodyI used this antibody in my previous lab and got pretty good data. I am doing a pioneer experiment in new lab now with NFKB research, hope to get small mount antibody for it.
Date published: 2017-08-01
Rated 5 out of 5 by from recommend this antibodyI had detected this antbody,I suggest loading 20ug sample, The antibody diluted 1:600 for WB analyisis for turbot intestine tissue .
Date published: 2017-07-18
Rated 5 out of 5 by from Good antibody.It works well in WB at dilution 1:500. I got clear band.
Date published: 2017-07-13
Rated 5 out of 5 by from GreatWe usually use 1:1000 for WB, It worked well.very nice
Date published: 2017-04-22
Rated 4 out of 5 by from GOOD RESULTWorks well for WB. Tested at 1:1000. Happy with the antibody.
Date published: 2017-03-08
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IKK alpha Antibody (B-8) is rated 4.8 out of 5 by 16.
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